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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

OGN Monoclonal antibody, PBS Only (Capture)

OGN Monoclonal Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2A5F11

Cat no : 66382-1-PBS

Synonyms

Osteoinductive factor, osteoglycin, OIF, OG, Mimecan

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of human artery tissue using OGN Monoclonal antibody (66382-1-Ig)

    WB analysis of human artery using 66382-1-Ig (same clone as 66382-1-PBS)

    Human artery tissue were subjected to SDS PAGE followed by western blot with 66382-1-Ig (OGN Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66382-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig artery tissue using OGN Monoclonal antibody (66382-1-Ig)

    WB analysis of pig artery using 66382-1-Ig (same clone as 66382-1-PBS)

    Pig artery tissue were subjected to SDS PAGE followed by western blot with 66382-1-Ig (OGN Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66382-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using OGN Monoclonal antibody (66382-1-Ig)

    IHC staining of human liver using 66382-1-Ig (same clone as 66382-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66382-1-Ig (OGN Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66382-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using OGN Monoclonal antibody (66382-1-Ig)

    IHC staining of human liver using 66382-1-Ig (same clone as 66382-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66382-1-Ig (OGN Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66382-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung tissue using OGN Monoclonal antibody (66382-1-Ig)

    IHC staining of human lung using 66382-1-Ig (same clone as 66382-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 66382-1-Ig (OGN Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66382-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung tissue using OGN Monoclonal antibody (66382-1-Ig)

    IHC staining of human lung using 66382-1-Ig (same clone as 66382-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 66382-1-Ig (OGN Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66382-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50723-1

    Cytometric bead array standard curve of MP50723-1

    Cytometric bead array standard curve of MP50723-1, OGN Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66382-1-PBS. Detection antibody: 66382-2-PBS. Standard:Ag3484. Range: 0.098-100 ng/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66382-1-PBS targets OGN as part of a matched antibody pair:

MP50723-1: 66382-1-PBS capture and 66382-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen OGN fusion protein Ag3484 相同性解析による交差性が予測される生物種
Full Name osteoglycin
Calculated molecular weight 298 aa, 34 kDa
Observed molecular weight 30-40 kDa
GenBank accession numberBC037273
Gene symbol OGN
Gene ID (NCBI) 4969
RRIDAB_2881758
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

This gene encodes a member of the small leucine-rich proteoglycan (SLRP) family of proteins. The encoded protein induces ectopic bone formation in conjunction with transforming growth factor beta and may regulate osteoblast differentiation. High expression of the encoded protein may be associated with elevated heart left ventricular mass. Alternative splicing results in multiple transcript variants which forms proteins with different molecular weight.