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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using OGT Monoclonal antibody (66823-1-Ig)

    WB analysis using 66823-1-Ig (same clone as 66823-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66823-1-Ig (OGT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66823-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using OGT Monoclonal antibody (66823-1-Ig)

    IHC staining of human lung cancer using 66823-1-Ig (same clone as 66823-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66823-1-Ig (OGT antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66823-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using OGT Monoclonal antibody (66823-1-Ig)

    IHC staining of human lung cancer using 66823-1-Ig (same clone as 66823-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66823-1-Ig (OGT antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66823-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using OGT Monoclonal antibody (66823-1-Ig)

    IHC staining of human colon cancer using 66823-1-Ig (same clone as 66823-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66823-1-Ig (OGT antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66823-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using OGT Monoclonal antibody (66823-1-Ig)

    IHC staining of human colon cancer using 66823-1-Ig (same clone as 66823-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66823-1-Ig (OGT antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66823-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using OGT Monoclonal antibody (66823-1-Ig)

    IF Staining of HepG2 using 66823-1-Ig (same clone as 66823-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using OGT antibody (66823-1-Ig, Clone: 2B2A6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 66823-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

OGT Monoclonal antibody, PBS Only

OGT Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66823-1-PBS
Clone No.2B2A6

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

FLJ23071, HRNT1, O GLCNAC, OGT

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66823-1-PBS targets OGT in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Reactivity Human, mouse, rat, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen OGT fusion protein Ag28402 相同性解析による交差性が予測される生物種
Full Name O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase)
Calculated molecular weight 1046 aa, 117 kDa
Observed molecular weight 110 kDa
GenBank accession numberBC014434
Gene Symbol OGT
Gene ID (NCBI) 8473
RRIDAB_2882166
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO15294
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

O-linked N-acetylglucosamine transferase (OGT) catalyzes the attachment of N-acetylglucosamine (GlcNAc) monosaccharides to the hydroxyl group of serine or threonine residues of numerous nuclear and cytoplasmic proteins and may play important roles in a large number of diverse intracellular processes ranging from translational control, transcription, transcriptional repression, INS resistance and regulation of the cell cycle. It exists as a heterotrimeric complex with two 110 kDa and one 70 kDa subunits. Recent studies have shown that O-GlcNAcylation plays essential roles in cancer formation and progression. O-GlcNAcylation as well as OGT expression was found to be significantly elevated in the cancer tissues.