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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using OGT Recombinant antibody (83113-1-RR)

    WB analysis using 83113-1-RR (same clone as 83113-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83113-1-RR (OGT antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83113-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using OGT Recombinant antibody (83113-1-RR)

    IF Staining of HeLa using 83113-1-RR (same clone as 83113-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using OGT antibody (83113-1-RR, Clone: 230385D11 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83113-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using OGT Recombinant antibody (83113-1-RR)

    FC experiment of HeLa using 83113-1-RR (same clone as 83113-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.2 ug OGT Recombinant antibody (83113-1-RR, Clone:230385D11)(red), or 0.2 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83113-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83113-1-RR

    Affinity and Kinetic Characterization of 83113-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83113-1-RR against Human OGT were performed. The affinity constant is below 1 pM.

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OGT Recombinant antibody, PBS Only

OGT Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 83113-1-PBS
Clone No.230385D11

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

O-linked N-acetylglucosamine transferase 110 kDa subunit, O-GlcNAc transferase subunit p110, O GLCNAC, HRNT1, EC:2.4.1.255

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83113-1-PBS targets OGT in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen OGT fusion protein Ag2160 相同性解析による交差性が予測される生物種
Full Name O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase)
Calculated molecular weight 1046 aa, 117 kDa
Observed molecular weight 110 kDa
GenBank accession numberBC014434
Gene Symbol OGT
Gene ID (NCBI) 8473
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO15294
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

O-linked N-acetylglucosamine transferase (OGT) catalyzes the attachment of N-acetylglucosamine (GlcNAc) monosaccharides to the hydroxyl group of serine or threonine residues of numerous nuclear and cytoplasmic proteins and may play important roles in a large number of diverse intracellular processes ranging from translational control, transcription, transcriptional repression, INS resistance and regulation of the cell cycle. It exists as a heterotrimeric complex with two 110 kDa and one 70 kDa subunits. Recent studies have shown that O-GlcNAcylation plays essential roles in cancer formation and progression. O-GlcNAcylation as well as OGT expression was found to be significantly elevated in the cancer tissues.