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Target

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Species Reactivity

Species Reactivity

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  • Featured Product
  • KD/KO Validated

OIP5 Monoclonal antibody

OIP5 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IP, CoIP, ELISA

Conjugate

Unconjugated

CloneNo.

1E9B5

Cat no : 66461-1-Ig

Synonyms

5730547N13Rik, Cancer/testis antigen 86, CT86, LINT 25, MIS18B, MIS18beta, OIP 5, OIP5, Opa interacting protein 5, Protein Mis18 beta

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using OIP5 Monoclonal antibody (66461-1-Ig)

    WB analysis using 66461-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66461-1-Ig (OIP5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using OIP5 Monoclonal antibody (66461-1-Ig)

    WB analysis of Jurkat using 66461-1-Ig

    WB result of OIP5 antibody (66461-1-Ig; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-OIP5 transfected Jurkat cells.

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Tested Applications

Positive WB detected inHeLa cells, Jurkat cells, COLO 320 cells, Raji cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66461-1-Ig targets OIP5 in WB, IP, CoIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen OIP5 fusion protein Ag2790 相同性解析による交差性が予測される生物種
Full Name Opa interacting protein 5
Calculated molecular weight 229 aa, 25 kDa
Observed molecular weight 25-30 kDa
GenBank accession numberBC015050
Gene symbol OIP5
Gene ID (NCBI) 11339
RRIDAB_2881830
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

OIP5, also named as IS18B and CT86, is required for recruitment of CENPA to centromeres and normal chromosome segregation during mitosis. It is over expressed in the majority of primary lung and esophageal cancers. OIP5 is a promising target for developing new prognostic biomarkers and anti-cancer drugs.

Protocols

Product Specific Protocols
WB protocol for OIP5 antibody 66461-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IP,CoIP

Oncogene

Engineered targeting OIP5 sensitizes bladder cancer to chemotherapy resistance via TRIP12-PPP1CB-YBX1 axis

Authors - Xianteng Wang
  • KD Validated
View Article