• Featured Product
  • KD/KO Validated

GTPBP9 Polyclonal antibody

GTPBP9 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 16371-1-AP

Synonyms

OLA1, Obg-like ATPase 1, Obg like ATPase 1, GTP-binding protein 9, GTP binding protein 9


☆対象製品が30%オフ!組換えタンパク質&免染用試薬 >>> キャンペーン実施中!


Tested Applications

Positive WB detected inJurkat cells, HepG2 cells, MCF-7 cells
Positive IHC detected inhuman liver cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16371-1-AP targets GTPBP9 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GTPBP9 fusion protein Ag9283 相同性解析による交差性が予測される生物種
Full Name Obg-like ATPase 1
Calculated molecular weight396aa,45 kDa; 278aa,31 kDa
Observed molecular weight 45 kDa
GenBank accession numberBC013925
Gene symbol OLA1
Gene ID (NCBI) 29789
RRIDAB_2157087
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for GTPBP9 antibody 16371-1-APDownload protocol
IHC protocol for GTPBP9 antibody 16371-1-APDownload protocol
IF protocol for GTPBP9 antibody 16371-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

bioRxiv

Obg-like ATPase 1 Genetic Deletion Leads to Dilated Cardiomyopathy in Mice and Structural Changes in Drosophila Heart

Authors - Praveen K Dubey
humanWB

Clin Exp Pharmacol Physiol

OBG-like ATPase 1 inhibition attenuates angiotensin II-induced hypertrophic response in human ventricular myocytes via GSK-3beta/beta-catenin signalling.

Authors - Gayathri Narasimhan
  • KD Validated
human,mouseWB

medRxiv

Identification and development of Tetra-ARMS PCR-based screening test for a genetic variant of OLA1 (Tyr254Cys) in the human failing heart

Authors - Praveen K Dubey
humanWB

PLoS One

Identification and development of Tetra-ARMS PCR-based screening test for a genetic variant of OLA1 (Tyr254Cys) in the human failing heart

Authors - Praveen K Dubey
humanWB

J Cell Sci

HIV-1 p17 matrix protein enhances type I interferon responses through the p17-OLA1-STING axis

Authors - Lianfei Zhang
  • KO Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

S (Verified Customer) (03-20-2023)

excellent

  • Applications: Western Blot
  • Primary Antibody Dilution: 1000
  • Cell Tissue Type: AC16
GTPBP9 Antibody Western Blot validation (1000 dilution) in AC16 (Cat no:16371-1-AP)
FH

p (Verified Customer) (05-28-2021)

Good

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:3000
  • Cell Tissue Type: H9C2
GTPBP9 Antibody Western Blot validation (1:3000 dilution) in H9C2 (Cat no:16371-1-AP)