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GTPBP9 Polyclonal antibody
GTPBP9 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
Cat no : 16371-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | Jurkat cells, HepG2 cells, MCF-7 cells |
Positive IHC detected in | human liver cancer tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 2 publications below |
WB | See 5 publications below |
IF | See 1 publications below |
Product Information
16371-1-AP targets GTPBP9 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | GTPBP9 fusion protein Ag9283 相同性解析による交差性が予測される生物種 |
Full Name | Obg-like ATPase 1 |
Calculated molecular weight | 396aa,45 kDa; 278aa,31 kDa |
Observed molecular weight | 45 kDa |
GenBank accession number | BC013925 |
Gene symbol | OLA1 |
Gene ID (NCBI) | 29789 |
RRID | AB_2157087 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
Product Specific Protocols | |
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WB protocol for GTPBP9 antibody 16371-1-AP | Download protocol |
IHC protocol for GTPBP9 antibody 16371-1-AP | Download protocol |
IF protocol for GTPBP9 antibody 16371-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
bioRxiv Obg-like ATPase 1 Genetic Deletion Leads to Dilated Cardiomyopathy in Mice and Structural Changes in Drosophila Heart | ||
Clin Exp Pharmacol Physiol OBG-like ATPase 1 inhibition attenuates angiotensin II-induced hypertrophic response in human ventricular myocytes via GSK-3beta/beta-catenin signalling.
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medRxiv Identification and development of Tetra-ARMS PCR-based screening test for a genetic variant of OLA1 (Tyr254Cys) in the human failing heart | ||
PLoS One Identification and development of Tetra-ARMS PCR-based screening test for a genetic variant of OLA1 (Tyr254Cys) in the human failing heart | ||
J Cell Sci HIV-1 p17 matrix protein enhances type I interferon responses through the p17-OLA1-STING axis
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Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH S (Verified Customer) (03-20-2023) | excellent
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FH p (Verified Customer) (05-28-2021) | Good
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