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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

OLFM3 Polyclonal antibody

OLFM3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 12596-1-AP

Synonyms

Olfactomedin 3, Olfactomedin-3, Noelin-3, NOELIN3, Noelin 3

Validation Data Gallery

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  • Western Blot (WB) analysis of PC-3 cells using OLFM3 Polyclonal antibody (12596-1-AP)

    WB analysis of PC-3 using 12596-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 12596-1-AP (OLFM3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A2780 cells using OLFM3 Polyclonal antibody (12596-1-AP)

    WB analysis of A2780 using 12596-1-AP

    A2780 cells were subjected to SDS PAGE followed by western blot with 12596-1-AP (OLFM3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human liver tissue using OLFM3 Polyclonal antibody (12596-1-AP)

    WB analysis of human liver using 12596-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 12596-1-AP (OLFM3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using OLFM3 Polyclonal antibody (12596-1-AP)

    WB analysis of NIH/3T3 using 12596-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12596-1-AP (OLFM3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using OLFM3 Polyclonal antibody (12596-1-AP)

    WB analysis of mouse liver using 12596-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 12596-1-AP (OLFM3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using OLFM3 Polyclonal antibody (12596-1-AP)

    IHC staining of human liver cancer using 12596-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12596-1-AP (OLFM3 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using OLFM3 Polyclonal antibody (12596-1-AP)

    IHC staining of human liver cancer using 12596-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12596-1-AP (OLFM3 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using OLFM3 Polyclonal antibody (12596-1-AP)

    IF Staining of HepG2 using 12596-1-AP

    Immunofluorescent analysis of HepG2 cells, using OLFM3 antibody 12596-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Tested Applications

Positive WB detected inPC-3 cells, A2780 cells, human liver tissue, mouse liver tissue, NIH/3T3 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12596-1-AP targets OLFM3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen OLFM3 fusion protein Ag3207 相同性解析による交差性が予測される生物種
Full Name olfactomedin 3
Calculated molecular weight 458 aa, 53 kDa
Observed molecular weight 50-55 kDa
GenBank accession numberBC022531
Gene symbol OLFM3
Gene ID (NCBI) 118427
RRIDAB_2157228
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for OLFM3 antibody 12596-1-APDownload protocol
IHC protocol for OLFM3 antibody 12596-1-APDownload protocol
IF protocol for OLFM3 antibody 12596-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Front Cell Dev Biol

Olfactomedin-3 Enhances Seizure Activity by Interacting With AMPA Receptors in Epilepsy Models.

Authors - Shirong Tang
View Article