Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse liver tissue |
| Positive IHC detected in | human liver cancer tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 4 publications below |
| IF | See 1 publications below |
Product Information
25137-1-AP targets ONECUT1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ONECUT1 fusion protein Ag18250 相同性解析による交差性が予測される生物種 |
| Full Name | one cut homeobox 1 |
| Calculated molecular weight | 465 aa, 51 kDa |
| Observed molecular weight | 51-55 kDa |
| GenBank accession number | BC140830 |
| Gene Symbol | ONECUT1 |
| Gene ID (NCBI) | 3175 |
| RRID | AB_2879918 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UBC0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ONECUT1, also named as Hepatocyte nuclear factor 6 (HNF6), is a 465 amino acid protein, which contains one CUT DNA-binding domain and one homeobox DNA-binding domain. ONECUT1 localizes in the nucleus and belongs to the CUT homeobox family. ONECUT1 as a transcription activator is involved in the some liver genes transcription. ONECUT1 is highly expressed in liver and lower in testis, skin.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ONECUT1 antibody 25137-1-AP | Download protocol |
| IHC protocol for ONECUT1 antibody 25137-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Virol Inhibition of Hepatitis B Virus Gene Expression and Replication by Hepatocyte Nuclear Factor 6. | ||
Virol Sin Psoralen inhibits hepatitis B viral replication by down-regulating the host transcriptional machinery of viral promoters | ||
bioRxiv Enhanced Plasmid-Based Transcriptional Activation in Developing Mouse Photoreceptors | ||
Cells The Zebrafish Retina and the Evolution of the Onecut-Mediated Pathway in Cell Type Differentiation |