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- KD/KO Validated
OPA1 Polyclonal antibody
OPA1 Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, rat, mouse and More (8)
Applications
WB, IHC, IF, IP, CoIP, ELISA
Conjugate
Unconjugated
138
Cat no : 27733-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | A431 cells, mouse brain tissue, HEK-293 cells, rat brain tissue, HEK-293T cells, HeLa cells, mouse liver tissue, HepG2 cells |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | mouse heart tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 132 publications below |
| IHC | See 4 publications below |
| IF | See 7 publications below |
| CoIP | See 1 publications below |
Product Information
27733-1-AP targets OPA1 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with Human, rat, mouse samples.
| Tested Reactivity | Human, rat, mouse |
| Cited Reactivity | human, mouse, rat, pig, chicken, zebrafish, bovine, hamster, goat, duck |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | OPA1 fusion protein Ag26887 相同性解析による交差性が予測される生物種 |
| Full Name | optic atrophy 1 (autosomal dominant) |
| Calculated molecular weight | 960 aa, 112 kDa |
| Observed molecular weight | 100 kDa, 80-90 kDa |
| GenBank accession number | BC075805 |
| Gene symbol | OPA1 |
| Gene ID (NCBI) | 4976 |
| RRID | AB_2810292 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
OPA1 is a nuclear-encoded mitochondrial protein with similarity to dynamin-related GTPases. OPA1 localizes to the inner mitochondrial membrane and helps regulate mitochondrial stability and energy output. This protein also sequesters cytochrome c. OPA1 is associated with the inner membrane and protects cells from apoptosis by regulating inner membrane dynamics. Mutation of OPA1 causes the disease dominant optic atrophy, a degeneration of the retinal ganglion cells. OPA1 undergoes complex posttranscriptional regulation and posttranslational proteolysis. OPA1 is regulated by proteolytic cleavage, which degrades long OPA1 isoforms into short isoforms. The gene OPA1 can be cleaved into some chains with MW 100 kDa and 80-90 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for OPA1 antibody 27733-1-AP | Download protocol |
| IHC protocol for OPA1 antibody 27733-1-AP | Download protocol |
| IP protocol for OPA1 antibody 27733-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Res Mitochondria-localized cGAS suppresses ferroptosis to promote cancer progression | ||
ACS Cent Sci Macrophage Inactivation by Small Molecule Wedelolactone via Targeting sEH for the Treatment of LPS-Induced Acute Lung Injury | ||
Mol Cell Serine synthesis sustains macrophage IL-1β production via NAD+-dependent protein acetylation | ||
Mol Cell Filamentous GLS1 promotes ROS-induced apoptosis upon glutamine deprivation via insufficient asparagine synthesis.
| ||
Acta Pharm Sin B Honokiol alleviated neurodegeneration by reducing oxidative stress and improving mitochondrial function in mutant SOD1 cellular and mouse models of amyotrophic lateral sclerosis | ||
J Hazard Mater Mitochondrial miR-12294-5p regulated copper-induced mitochondrial oxidative stress and mitochondrial quality control imbalance by targeted inhibition of CISD1 in chicken livers |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH P (Verified Customer) (09-23-2024) | excellent!
 |
FH Mi (Verified Customer) (02-21-2023) | Works well in human adipocyte cells, we got clean bands at the expected size.
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