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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using OPA1 Polyclonal antibody (32465-1-AP)

    WB analysis using 32465-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32465-1-AP (OPA1 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using OPA1 Polyclonal antibody (32465-1-AP)

    IP experiment of mouse brain using 32465-1-AP

    IP result of anti-OPA1 (IP:32465-1-AP, 4ug; Detection:32465-1-AP 1:40000) with mouse brain tissue lysate 1840 ug.

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OPA1 Polyclonal antibody

OPA1 Polyclonal Antibody for WB, IP, ELISA
Cat No. 32465-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Synonyms

OPA1 gene, OPA 1, EC:3.6.5.5, Dynamin-like GTPase OPA1, short form, Dynamin-like GTPase OPA1, mitochondrial

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA431 cells, HeLa cells, HepG2 cells, mouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

32465-1-AP targets OPA1 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen OPA1 fusion protein Ag38295 相同性解析による交差性が予測される生物種
Full Name optic atrophy 1 (autosomal dominant)
Calculated molecular weight 960 aa, 112 kDa
Observed molecular weight80-100 kDa
GenBank accession numberBC075805
Gene Symbol OPA1
Gene ID (NCBI) 4976
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDO60313
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

OPA1 is a nuclear-encoded mitochondrial protein with similarity to dynamin-related GTPases. OPA1 localizes to the inner mitochondrial membrane and helps regulate mitochondrial stability and energy output. This protein also sequesters cytochrome c. OPA1 is associated with the inner membrane and protects cells from apoptosis by regulating inner membrane dynamics. Mutation of OPA1 causes the disease dominant optic atrophy, a degeneration of the retinal ganglion cells. OPA1 undergoes complex posttranscriptional regulation and posttranslational proteolysis. OPA1 is regulated by proteolytic cleavage, which degrades long OPA1 isoforms into short isoforms.

Protocols

Product Specific Protocols
WB protocol for OPA1 antibody 32465-1-APDownload protocol
IP protocol for OPA1 antibody 32465-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols