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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Oligophrenin 1 Polyclonal antibody

Oligophrenin 1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17445-1-AP

Synonyms

MRX60, oligophrenin 1, OPHN1, OPN1

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using Oligophrenin 1 Polyclonal antibody (17445-1-AP)

    WB analysis using 17445-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17445-1-AP (Oligophrenin 1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using Oligophrenin 1 Polyclonal antibody (17445-1-AP)

    WB analysis of mouse skeletal muscle using 17445-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 17445-1-AP (Oligophrenin 1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using Oligophrenin 1 Polyclonal antibody (17445-1-AP)

    IP experiment of mouse brain using 17445-1-AP

    IP result of anti-Oligophrenin 1 (IP:17445-1-AP, 4ug; Detection:17445-1-AP 1:500) with mouse brain tissue lysate 5200ug.

  • Immunohistochemistry (IHC) staining of human colon tissue using Oligophrenin 1 Polyclonal antibody (17445-1-AP)

    IHC staining of human colon using 17445-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 17445-1-AP (Oligophrenin 1 antibody at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using Oligophrenin 1 Polyclonal antibody (17445-1-AP)

    IHC staining of human colon using 17445-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 17445-1-AP (Oligophrenin 1 antibody at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using Oligophrenin 1 Polyclonal antibody (17445-1-AP)

    IHC staining of human stomach cancer using 17445-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 17445-1-AP (Oligophrenin 1 antibody at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using Oligophrenin 1 Polyclonal antibody (17445-1-AP)

    IHC staining of human stomach cancer using 17445-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 17445-1-AP (Oligophrenin 1 antibody at dilution of 1:50 (under 40x lens).

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Tested Applications

Positive WB detected inmouse brain tissue, mouse skeletal muscle tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman colon tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

17445-1-AP targets Oligophrenin 1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Oligophrenin 1 fusion protein Ag11538 相同性解析による交差性が予測される生物種
Full Name oligophrenin 1
Calculated molecular weight 802 aa, 92 kDa
Observed molecular weight 91 kDa
GenBank accession numberBC059393
Gene symbol Oligophrenin 1
Gene ID (NCBI) 4983
RRIDAB_2158304
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The OPHN1 gene encodes a Rho-GTPase activating protein (RhoGAP), and mutations in OPHN1 are responsible for non-specific X-linked mental retardation (NSMR). OPHN1 is highly expressed in the brain and present both pre- and postsynaptically in neurons.

Protocols

Product Specific Protocols
WB protocol for Oligophrenin 1 antibody 17445-1-APDownload protocol
IHC protocol for Oligophrenin 1 antibody 17445-1-APDownload protocol
IP protocol for Oligophrenin 1 antibody 17445-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell Rep

Error-prone protein synthesis recapitulates early symptoms of Alzheimer disease in aging mice

Authors - Margarita Brilkova
View Article