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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

OPN, SPP1 Recombinant antibody, PBS Only

OPN, SPP1 Recombinant Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, rat, mouse

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

6B11

Cat no : 80912-4-PBS

Synonyms

BNSP, Bone sialoprotein 1, BSPI, ETA 1, Nephropontin, OPN, Osteopontin, secreted phosphoprotein 1, SPP 1, SPP1, Urinary stone protein, Uropontin

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using OPN, SPP1 Recombinant antibody (80912-4-RR)

    WB analysis using 80912-4-RR (same clone as 80912-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80912-4-RR (OPN, SPP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80912-4-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

80912-4-PBS targets OPN, SPP1 in WB, Indirect ELISA applications and shows reactivity with Human, rat, mouse samples.

Tested Reactivity Human, rat, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen OPN, SPP1 fusion protein Ag19216 相同性解析による交差性が予測される生物種
Full Name secreted phosphoprotein 1
Calculated molecular weight 314 aa, 35 kDa
Observed molecular weight 70 kDa, 44-66 kDa
GenBank accession numberBC007016
Gene symbol SPP1
Gene ID (NCBI) 6696
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Osteopontin (OPN), also known as SPP1, is a secreted glycophosphoprotein that belongs to the small integrin-binding ligand N-linked glycoprotein (SIBLING) family. Originally isolated from bone, OPN has been found in kidneys, vascular tissues, biological fluids, and various tumor tissues (PMID: 15138464; 16406521). OPN can interact with CD44 and integrins and regulate diverse biological processes. It has a multifaceted role in bone development and remodeling, and is also involved in the inflammatory and immune response, oncogenesis and cancer progression. The very acidic nature of OPN, as well as the presence of variable posttranslational modifications, has led to anomalous migration in SDS-polyacrylamide gels and therefore to reports of different molecular weights for OPN (PMID: 8293561). Depending on the cell and tissue source and/or the SDS-PAGE system, OPN migrates with a molecular weight of 44-80 kDa, as well as at some smaller bands corresponding to peptide fragments (PMID: 8195113; 17890765).