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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse eye tissue using OPN1SW Recombinant antibody (83754-6-RR)

    IHC staining of mouse eye using 83754-6-RR (same clone as 83754-6-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 83754-6-RR (OPN1SW antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83754-6-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse eye tissue using OPN1SW Recombinant antibody (83754-6-RR)

    IF Staining of mouse eye using 83754-6-RR (same clone as 83754-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using OPN1SW antibody (83754-6-RR, Clone: 240714A5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83754-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83754-6-RR

    Affinity and Kinetic Characterization of 83754-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83754-6-RR against Human OPN1SW were performed. The affinity constant is 3.58 nM.

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OPN1SW Recombinant antibody, PBS Only

OPN1SW Uni-rAbTM Recombinant Antibody for IHC, IF/ICC, Indirect ELISA
Cat No. 83754-6-PBS
Clone No.240714A5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, Indirect ELISA

Synonyms

Short-wave-sensitive opsin 1, CBT, BOP, Blue-sensitive opsin, Blue sensitive opsin

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83754-6-PBS targets OPN1SW in IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen OPN1SW fusion protein Ag20445 相同性解析による交差性が予測される生物種
Full Name opsin 1 (cone pigments), short-wave-sensitive
Calculated molecular weight 348 aa, 39 kDa
GenBank accession numberBC156719
Gene Symbol OPN1SW
Gene ID (NCBI) 611
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP03999
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

OPN1SW (Short-wave-sensitive opsin 1) belongs to the G-protein coupled receptor 1 family, opsin subfamily. It's one of three types of cone photoreceptors responsible for normal color vision. Inherited tritan color vision deficiencies exhibit an autosomal dominant inheritance pattern and are caused by mutations in OPN1SW (PMID: 32400513).