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OPTN 抗体 68 文献

Rabbit Polyclonal| カタログ番号: 10837-1-AP

注目製品 KD/KO 検証済

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Related Primary Antibodies

適用アプリケーション

アプリケーション:
WB, IP, IHC, IF, FC, ELISA
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OPTN Antibody WB HeLa cells 10837-1-AP

various lysates were subjected to SDS PAGE followed by western blot with 10837-1-AP (OPTN antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

OPTN Antibody IP mouse brain tissue 10837-1-AP

IP Result of anti-OPTN (IP:10837-1-AP, 3ug; Detection:10837-1-AP 1:800) with mouse brain tissue lysate 8000ug.

OPTN Antibody FC HeLa cells 10837-1-AP

1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human OPTN (10837-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

OPTN Antibody IHC human gliomas tissue 10837-1-AP

Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10837-1-AP (OPTN Antibody) at dilution of 1:200 (under 10x lens).

OPTN Antibody IHC human gliomas tissue 10837-1-AP

Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10837-1-AP (OPTN Antibody) at dilution of 1:200 (under 40x lens).

種特異性:
human, mouse, rat, pig
WB ポジティブ:
HeLa cells, mouse testis tissue, mouse brain tissue
IP ポジティブ:
mouse brain tissue
IHC ポジティブ:
human gliomas tissue
FC ポジティブ:
HeLa cells
推奨希釈倍率:
WB : 1:2000-1:12000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IHC : 1:50-1:500

Protocols

Product-specific protocols

Standard Protocols

製品情報

由来:
Rabbit
精製方法:
Antigen affinity purification
アイソタイプ:
IgG
バッファー/保存方法:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

ターゲット情報

免疫原:
OPTN fusion protein Ag1272
正式名:
optineurin
計算分子量:
66 kDa
検出分子量:
66 kDa, 70-74 kDa
GenBank accession number:
BC013876
Gene ID (NCBI):
10133
遺伝子シンボル:
OPTN
別名:
E3 14.7K interacting protein, FIP 2, FIP2, GLC1E, HIP 7, HIP7, Huntingtin yeast partner L, HYPL, NEMO related protein, NRP, optineurin, OPTN, TFIIIA INTP

背景説明

OPTN, also named as FIP2, GLC1E, HIP7, HYPL and NRP, plays a neuroprotective role in the eye and optic nerve. It is probably part of the TNF-alpha signaling pathway that can shift the equilibrium toward induction of cell death. OPTN may act by regulating membrane trafficking and cellular morphogenesis via a complex that contains Rab8 and hungtingtin (HD). OPTN may constitute a cellular target for adenovirus E3 14.7, an inhibitor of TNF-alpha functions, thereby affecting cell death. Defects in OPTN are the cause of primary open angle glaucoma type 1E (GLC1E). Defects in OPTN are a cause of susceptibility to normal pressure glaucoma (NPG). OPTN mutated in adult-onset primary open angle glaucoma. It supports the protective role of OPTN in the trabecular meshwork. OPTN has 3 isoforms with MW 66,65 and 60 kDa (refer to UniProt). Catalog#10837-1-AP recognises 66 and 70-74 kDa band, and the additional 70-74 kDa band due to phosphorylation.


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