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  • Featured Product
  • KD/KO Validated

OPTN Monoclonal antibody, PBS Only

OPTN Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

6C1H4

Cat no : 60293-1-PBS

Synonyms

AAT4, E3 14.7K interacting protein, FAA4, FIP 2, FIP2, GLC1E, HIP 7, HIP7, Huntingtin yeast partner L, HYPL, NEMO related protein, NRP, optineurin, OPTN

Validation Data Gallery

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  • Western Blot (WB) analysis of human heart tissue using OPTN Monoclonal antibody (60293-1-Ig)

    WB analysis of human heart using 60293-1-Ig (same clone as 60293-1-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 60293-1-Ig (OPTN Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60293-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using OPTN Monoclonal antibody (60293-1-Ig)

    IHC staining of human pancreas cancer using 60293-1-Ig (same clone as 60293-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 60293-1-Ig (OPTN Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 60293-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using OPTN Monoclonal antibody (60293-1-Ig)

    IHC staining of human pancreas cancer using 60293-1-Ig (same clone as 60293-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 60293-1-Ig (OPTN Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 60293-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using OPTN Monoclonal antibody (60293-1-Ig)

    IHC staining of human brain using 60293-1-Ig (same clone as 60293-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 60293-1-Ig (OPTN Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 60293-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using OPTN Monoclonal antibody (60293-1-Ig)

    IHC staining of human brain using 60293-1-Ig (same clone as 60293-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 60293-1-Ig (OPTN Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 60293-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using OPTN Monoclonal antibody (60293-1-Ig)

    IF Staining of HepG2 using 60293-1-Ig (same clone as 60293-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 60293-1-Ig (OPTN antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60293-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60293-1-PBS targets OPTN in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen OPTN fusion protein Ag1272 相同性解析による交差性が予測される生物種
Full Name optineurin
Calculated molecular weight 66 kDa
Observed molecular weight 70-74 kDa
GenBank accession numberBC013876
Gene symbol OPTN
Gene ID (NCBI) 10133
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

OPTN, also named as FIP2, GLC1E, HIP7, HYPL and NRP, plays a neuroprotective role in the eye and optic nerve. It is probably part of the TNF-alpha signaling pathway that can shift the equilibrium toward induction of cell death. OPTN may act by regulating membrane trafficking and cellular morphogenesis via a complex that contains Rab8 and hungtingtin (HD). OPTN may constitute a cellular target for adenovirus E3 14.7, an inhibitor of TNF-alpha functions, thereby affecting cell death. Defects in OPTN are the cause of primary open angle glaucoma type 1E (GLC1E). Defects in OPTN are a cause of susceptibility to normal pressure glaucoma (NPG). OPTN mutated in adult-onset primary open angle glaucoma. It supports the protective role of OPTN in the trabecular meshwork.