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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

OPTN/Optineurin Recombinant antibody, PBS Only

OPTN/Optineurin Recombinant Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240636A7

Cat no : 83634-5-PBS

Synonyms

Optineurin, OPTN, FIP2, FIP 2, FAA4

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using OPTN/Optineurin Recombinant antibody (83634-5-RR)

    WB analysis using 83634-5-RR (same clone as 83634-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83634-5-RR (OPTN antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83634-5-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using OPTN/Optineurin Recombinant antibody (83634-5-RR)

    WB analysis of HeLa using 83634-5-RR (same clone as 83634-5-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 83634-5-RR (OPTN antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83634-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using OPTN/Optineurin Recombinant antibody (83634-5-RR)

    IHC staining of human gliomas using 83634-5-RR (same clone as 83634-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 83634-5-RR (OPTN antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83634-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using OPTN/Optineurin Recombinant antibody (83634-5-RR)

    IHC staining of human gliomas using 83634-5-RR (same clone as 83634-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 83634-5-RR (OPTN antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83634-5-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using OPTN/Optineurin Recombinant antibody (83634-5-RR)

    FC experiment of HeLa using 83634-5-RR (same clone as 83634-5-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug OPTN Recombinant antibody (83634-5-RR, Clone:240636A7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83634-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83634-5-RR

    Affinity and Kinetic Characterization of 83634-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83634-5-RR against Human OPTN were performed. The affinity constant is 12.2 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83634-5-PBS targets OPTN/Optineurin in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen OPTN/Optineurin fusion protein Ag1272 相同性解析による交差性が予測される生物種
Full Name optineurin
Calculated molecular weight 66 kDa
Observed molecular weight 66-70 kDa
GenBank accession numberBC013876
Gene symbol OPTN
Gene ID (NCBI) 10133
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

OPTN, also named as FIP2, GLC1E, HIP7, HYPL, and NRP, plays a neuroprotective role in the eye and optic nerve. It is probably part of the TNF-alpha signaling pathway that can shift the equilibrium toward induction of cell death. OPTN may act by regulating membrane trafficking and cellular morphogenesis via a complex that contains Rab8 and hungtingtin (HD). OPTN may constitute a cellular target for adenovirus E3 14.7, an inhibitor of TNF-alpha functions, thereby affecting cell death. Defects in OPTN cause susceptibility to normal pressure glaucoma (NPG). OPTN mutated in adult-onset primary open angle glaucoma. It supports the protective role of OPTN in the trabecular meshwork.