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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ORAI2 Polyclonal antibody, PBS Only

ORAI2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 20592-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using ORAI2 Polyclonal antibody (20592-1-AP)

    WB analysis of MCF-7 using 20592-1-AP (same clone as 20592-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 20592-1-AP (ORAI2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20592-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse heart tissue using ORAI2 Polyclonal antibody (20592-1-AP)

    WB analysis of mouse heart using 20592-1-AP (same clone as 20592-1-PBS)

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 20592-1-AP (ORAI2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20592-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using ORAI2 Polyclonal antibody (20592-1-AP)

    IHC staining of human kidney using 20592-1-AP (same clone as 20592-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney slide using 20592-1-AP (ORAI2 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 20592-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using ORAI2 Polyclonal antibody (20592-1-AP)

    IF Staining of HEK-293 using 20592-1-AP (same clone as 20592-1-PBS)

    Immunofluorescent analysis of HEK-293 cells using 20592-1-AP (ORAI2 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG. This data was developed using the same antibody clone with 20592-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

20592-1-PBS targets ORAI2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ORAI2 fusion protein Ag14540 相同性解析による交差性が予測される生物種
Full Name ORAI calcium release-activated calcium modulator 2
Calculated molecular weight 254 aa, 29 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC069270
Gene symbol ORAI2
Gene ID (NCBI) 80228
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

ORAI2, also named as C7orf19, CBCIP2, TMEM142B and PP1729, is a Ca2+ release-activated Ca2+-like (CRAC-like) channel subunit. The MW of ORAI2 is about 36 kDa for glycosylation (PMID: 19675303).