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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ORAI3 Recombinant antibody

ORAI3 Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

241568A2

Cat no : 84340-2-RR

Synonyms

Transmembrane protein 142C, TMEM142C, Protein orai-3, 241568A2

Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using ORAI3 Recombinant antibody (84340-2-RR)

    WB analysis of MCF-7 using 84340-2-RR

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 84340-2-RR (ORAI3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using ORAI3 Recombinant antibody (84340-2-RR)

    WB analysis using 84340-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84340-2-RR (ORAI3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using ORAI3 Recombinant antibody (84340-2-RR)

    IF Staining of Jurkat using 84340-2-RR

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using ORAI3 antibody (84340-2-RR, Clone: 241568A2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84340-2-RR

    Affinity and Kinetic Characterization of 84340-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84340-2-RR against Human ORAI3 were performed. The affinity constant is 1.24 nM.

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Tested Applications

Positive WB detected inJurkat cells, MCF-7 cells, A549 cells
Positive IF/ICC detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84340-2-RR is ORAI3 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ORAI3 fusion protein Ag36135 相同性解析による交差性が予測される生物種
Full Name ORAI calcium release-activated calcium modulator 3
Calculated molecular weight 295 aa, 32 kDa
Observed molecular weight ~30 kDa，~60 kDa
GenBank accession numberBC006126
Gene symbol ORAI3
Gene ID (NCBI) 93129
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ORAI3, also named TMEM142C, is a key regulator or component of store-operated Ca2+ channels and transcription factor NFAT nuclear import. ORAI3 channels appeared to differ from Orai1 and -2 in being somewhat resistant to Ca2+ depotentiation. The molecular weight of monomers and dimers for Orai3 is about 30-35 kDa and 60-70 kDa (PMID: 20971921; 17293345; 22580508).

Protocols

Product Specific Protocols
WB protocol for ORAI3 antibody 84340-2-RRDownload protocol
IF protocol for ORAI3 antibody 84340-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols