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Validation Data Gallery

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  • Western Blot (WB) analysis of human plasma using ORM2-Specific Monoclonal antibody (66217-1-Ig)

    WB analysis of human plasma using 66217-1-Ig (same clone as 66217-1-PBS)

    human plasma were subjected to SDS PAGE followed by western blot with 66217-1-Ig (ORM2-Specific antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66217-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Recombinant protein using ORM2-Specific Monoclonal antibody (66217-1-Ig)

    WB analysis of Recombinant protein using 66217-1-Ig (same clone as 66217-1-PBS)

    Recombinant protein of ORM1 and ORM2 were subjected to SDS PAGE followed by western blot with 66217-1-Ig (ORM2-Specific antibody at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66217-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using ORM2-Specific Monoclonal antibody (66217-1-Ig)

    WB analysis of A549 using 66217-1-Ig (same clone as 66217-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 66217-1-Ig (ORM2-Specific antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66217-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ORM2-Specific Monoclonal antibody (66217-1-Ig)

    IHC staining of human liver cancer using 66217-1-Ig (same clone as 66217-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66217-1-Ig (ORM2-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66217-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ORM2-Specific Monoclonal antibody (66217-1-Ig)

    IHC staining of human liver cancer using 66217-1-Ig (same clone as 66217-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66217-1-Ig (ORM2-Specific antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66217-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using ORM2-Specific Monoclonal antibody (66217-1-Ig)

    IHC staining of human colon cancer using 66217-1-Ig (same clone as 66217-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66217-1-Ig (ORM2-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66217-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using ORM2-Specific Monoclonal antibody (66217-1-Ig)

    IHC staining of human colon cancer using 66217-1-Ig (same clone as 66217-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66217-1-Ig (ORM2-Specific antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66217-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ORM2-Specific Monoclonal antibody (66217-1-Ig)

    IF Staining of HepG2 using 66217-1-Ig (same clone as 66217-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ORM2-Specific antibody (66217-1-Ig, Clone: 4E9H10 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66217-1-PBS in a different storage buffer formulation.

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ORM2-Specific Monoclonal antibody, PBS Only

ORM2-Specific Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66217-1-PBS
Clone No.4E9H10

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

ORM1/2, ORM2, AGPB, AGP2, AGP B'

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66217-1-PBS targets ORM2-Specific in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ORM2-Specific fusion protein Ag18984 相同性解析による交差性が予測される生物種
Full Name orosomucoid 2
Calculated molecular weight 24 kDa
Observed molecular weight 45 kDa
GenBank accession numberBC015964
Gene Symbol ORM2
Gene ID (NCBI) 5005
RRIDAB_2881608
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP19652
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

ORM2, also named as AGP2 and OMD 2, belongs to the calycin superfamily and Lipocalin family. It functions as transport protein in the blood stream. ORM2 binds various hydrophobic ligands in the interior of its beta-barrel domain. It also binds synthetic drugs and influences their distribution and availability. ORM2 appears to function in modulating the activity of the immune system during the acute-phase reaction. ORM2 is one of the conserved endoplasmic reticulum membrane proteins which regulating lipid homeostasis and protein quality control. This antibody can specially recognize ORM2. It recognizes a band about 45 kDa in human plasma which may be due to the glycosylation of ORM2 or the dimer formation of the protein.