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Species Reactivity
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

OS9 Recombinant antibody, PBS Only

OS9 Recombinant Antibody for WB, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230149B4

Cat no : 82932-1-PBS

Synonyms

Protein OS-9, Amplified in osteosarcoma 9, 230149B4

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using OS9 Recombinant antibody (82932-1-RR)

    WB analysis using 82932-1-RR (same clone as 82932-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82932-1-RR (OS9 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82932-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using OS9 Recombinant antibody (82932-1-RR)

    FC experiment of HeLa using 82932-1-RR (same clone as 82932-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug OS9 Recombinant antibody (82932-1-RR, Clone:230149B4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82932-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82932-1-RR

    Affinity and Kinetic Characterization of 82932-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82932-1-RR against Human OS9 were performed. The affinity constant is below 1 pM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82932-1-PBS targets OS9 in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen OS9 fusion protein Ag0106 相同性解析による交差性が予測される生物種
Full Name osteosarcoma amplified 9, endoplasmic reticulum associated protein
Calculated molecular weight 76 kDa
Observed molecular weight83-97 kDa
GenBank accession numberBC000532
Gene symbol OS9
Gene ID (NCBI) 10956
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

OS-9 is a ubiquitously expressed ensoplasmic reticulum (ER)-associated protein originally identified as being amplified in certain osteosarcomas. It functions in ER quality control and ER-associated degradation (ERAD). There are three isoforms of this protein: OS-9.1, OS-9.2 and OS-9.3. The longest isoform, OS-9.1, contains 667 aa, OS-9.2 lacks aa 535-589, whereas OS-9.3 lacks aa 456-470 and 535-589. OS-9.1 and OS-9.2 are N-glycosylated, ubiquitously expressed in human tissues, and amplified in tumors.