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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

OS9 Recombinant antibody

OS9 Recombinant Antibody for IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230149C6

Cat no : 82932-2-RR

Synonyms

Protein OS-9, Amplified in osteosarcoma 9, 230149C6

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse testis tissue using OS9 Recombinant antibody (82932-2-RR)

    IHC staining of mouse testis using 82932-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 82932-2-RR (OS9 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using OS9 Recombinant antibody (82932-2-RR)

    IHC staining of mouse testis using 82932-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 82932-2-RR (OS9 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using OS9 Recombinant antibody (82932-2-RR)

    IF Staining of HeLa using 82932-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using OS9 antibody (82932-2-RR, Clone: 230149C6 ) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Flow cytometry (FC) experiment of HeLa cells using OS9 Recombinant antibody (82932-2-RR)

    FC experiment of HeLa using 82932-2-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug OS9 Recombinant antibody (82932-2-RR, Clone:230149C6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 82932-2-RR

    Affinity and Kinetic Characterization of 82932-2-RR

    Biolayer interferometry (BLl) kinetic assays of 82932-2-RR against Human OS9 were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82932-2-RR targets OS9 in IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen OS9 fusion protein Ag0106 相同性解析による交差性が予測される生物種
Full Name osteosarcoma amplified 9, endoplasmic reticulum associated protein
Calculated molecular weight 76 kDa
GenBank accession numberBC000532
Gene symbol OS9
Gene ID (NCBI) 10956
RRIDAB_3670674
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

OS-9 is a ubiquitously expressed ensoplasmic reticulum (ER)-associated protein originally identified as being amplified in certain osteosarcomas. It functions in ER quality control and ER-associated degradation (ERAD). There are three isoforms of this protein: OS-9.1, OS-9.2 and OS-9.3. The longest isoform, OS-9.1, contains 667 aa, OS-9.2 lacks aa 535-589, whereas OS-9.3 lacks aa 456-470 and 535-589. OS-9.1 and OS-9.2 are N-glycosylated, ubiquitously expressed in human tissues, and amplified in tumors.

Protocols

Product Specific Protocols
IHC protocol for OS9 antibody 82932-2-RRDownload protocol
IF protocol for OS9 antibody 82932-2-RRDownload protocol
FC protocol for OS9 antibody 82932-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols