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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

OSBPL11 Polyclonal antibody

OSBPL11 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 11318-1-AP

Synonyms

ORP 11, ORP11, OSBP related protein 11, OSBP12, OSBPL11, TCCCIA00292

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using OSBPL11 Polyclonal antibody (11318-1-AP)

    WB analysis using 11318-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11318-1-AP (OSBPL11 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse testis tissue using OSBPL11 Polyclonal antibody (11318-1-AP)

    IP experiment of mouse testis using 11318-1-AP

    IP result of anti-OSBPL11 (IP:11318-1-AP, 4ug; Detection:11318-1-AP 1:300) with mouse testis tissue lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using OSBPL11 Polyclonal antibody (11318-1-AP)

    IHC staining of human ovary tumor using 11318-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 11318-1-AP (OSBPL11 antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

Positive WB detected inHeLa cells, HepG2 cells
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11318-1-AP targets OSBPL11 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen OSBPL11 fusion protein Ag1855 相同性解析による交差性が予測される生物種
Full Name oxysterol binding protein-like 11
Calculated molecular weight 747 aa, 84 kDa
Observed molecular weight 84 kDa
GenBank accession numberBC010422
Gene symbol OSBPL11
Gene ID (NCBI) 114885
RRIDAB_2267735
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for OSBPL11 antibody 11318-1-APDownload protocol
IHC protocol for OSBPL11 antibody 11318-1-APDownload protocol
IP protocol for OSBPL11 antibody 11318-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human

Elife

The ORP9-ORP11 dimer promotes sphingomyelin synthesis

Authors - Birol Cabukusta
View Article