Validation Data Gallery

Filter: AllWBIHC

WB analysis of human brain using 11879-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 11879-1-AP (OSBPL9 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 11879-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 11879-1-AP (OSBPL9 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of human testis using 11879-1-AP
human testis tissue were subjected to SDS PAGE followed by western blot with 11879-1-AP (OSBPL9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse lung using 11879-1-AP
mouse lung tissue were subjected to SDS PAGE followed by western blot with 11879-1-AP (OSBPL9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of human testis using 11879-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 11879-1-AP (OSBPL9 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human testis using 11879-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 11879-1-AP (OSBPL9 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human colon cancer using 11879-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer using 11879-1-AP (OSBPL9 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human colon cancer using 11879-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer using 11879-1-AP (OSBPL9 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human colon cancer using 11879-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer using 11879-1-AP (OSBPL9 antibody) at dilution of 1:50 (under 40x lens).

OSBPL9 Polyclonal antibody

OSBPL9 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 11879-1-AP

Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

ORP 9, ORP9, OSBP related protein 9, OSBP4, OSBPL9

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (4)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	human brain tissue, human testis tissue, mouse lung tissue
Positive IHC detected in	human testis tissue, human colon cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 3 publications below

Product Information

11879-1-AP targets OSBPL9 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	OSBPL9 fusion protein Ag2476 相同性解析による交差性が予測される生物種
Full Name	oxysterol binding protein-like 9
Calculated molecular weight	736 aa, 83 kDa
Observed molecular weight	90 kDa
GenBank accession number	BC025978
Gene Symbol	OSBPL9
Gene ID (NCBI)	114883
RRID	AB_10638005
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q96SU4
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

ORP9 is expressed as a full-length (ORP9L) or truncated version missing the PH domain (ORP9S).

Protocols

Product Specific Protocols
WB protocol for OSBPL9 antibody 11879-1-AP	Download protocol
IHC protocol for OSBPL9 antibody 11879-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nature A phosphoinositide signalling pathway mediates rapid lysosomal repair Authors - Jay Xiaojun Tan View Article
human	WB	Cell Mol Life Sci ORP9 and ORP10 form a heterocomplex to transfer phosphatidylinositol 4-phosphate at ER-TGN contact sites Authors - Ruyue He View Article
human	WB	J Cell Biol Phosphatidylserine regulates plasma membrane repair through tetraspanin-enriched macrodomains Authors - Yang E Li KO Validated View Article
human		Elife The ORP9-ORP11 dimer promotes sphingomyelin synthesis Authors - Birol Cabukusta View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of human brain using 11879-1-AP

WB analysis of human brain using 11879-1-AP

WB analysis of human testis using 11879-1-AP

WB analysis of mouse lung using 11879-1-AP

IHC staining of human testis using 11879-1-AP

IHC staining of human testis using 11879-1-AP

IHC staining of human colon cancer using 11879-1-AP

IHC staining of human colon cancer using 11879-1-AP

IHC staining of human colon cancer using 11879-1-AP

OSBPL9 Polyclonal antibody

OSBPL9 Polyclonal Antibody for WB, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

A phosphoinositide signalling pathway mediates rapid lysosomal repair

ORP9 and ORP10 form a heterocomplex to transfer phosphatidylinositol 4-phosphate at ER-TGN contact sites

Phosphatidylserine regulates plasma membrane repair through tetraspanin-enriched macrodomains

The ORP9-ORP11 dimer promotes sphingomyelin synthesis