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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using OSCAR Polyclonal antibody (21996-1-AP)

    WB analysis using 21996-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21996-1-AP (OSCAR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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OSCAR Polyclonal antibody

OSCAR Polyclonal Antibody for WB, ELISA
Cat No. 21996-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Poly-Ig receptor 3, PIgR-3, PIgR3, Osteoclast-associated receptor, Osteoclast-associated immunoglobulin-like receptor

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inHL-60 cells, THP-1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21996-1-AP targets OSCAR in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen OSCAR fusion protein Ag17366 相同性解析による交差性が予測される生物種
Full Name osteoclast associated, immunoglobulin-like receptor
Calculated molecular weight 282 aa, 31 kDa
Observed molecular weight40 kDa
GenBank accession numberBC035023
Gene Symbol OSCAR
Gene ID (NCBI) 126014
RRIDAB_3669383
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IYS5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Osteoclast-associated receptor (OSCAR) is an FcRγ-associated receptor that belongs to the leukocyte receptor cluster (LRC). OSCAR is expressed by myeloid cells and is involved in antigen presentation and activation of human dendritic cells(PMID: 15155468; PMID: 15650060). OSCAR is expressed in osteoclast cells and involved in the differentiation of osteoclasts from peripheral blood mononuclear cells (PBMC). The N-glycosylated form of human OSCAR has a molecular weight of approximately 40-45 kDa, while 30 kDa corresponds to its fully non-glycosylated form (PMID: 15155468; 24902903; 27917924)

Protocols

Product Specific Protocols
WB protocol for OSCAR antibody 21996-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols