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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using OSMR Recombinant antibody (84555-1-RR)

    WB analysis using 84555-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84555-1-RR (OSMR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A375 cells using OSMR Recombinant antibody (84555-1-RR)

    WB analysis of A375 using 84555-1-RR

    A375 cells were subjected to SDS PAGE followed by western blot with 84555-1-RR (OSMR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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OSMR Recombinant antibody

OSMR Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 84555-1-RR
Clone No.241865A6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Interleukin-31 receptor subunit beta, IL-31R-beta, IL-31RB, IL-31R subunit beta, IL-31 receptor subunit beta

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, A375 cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84555-1-RR targets OSMR in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen OSMR fusion protein Ag1426 相同性解析による交差性が予測される生物種
Full Name oncostatin M receptor
Calculated molecular weight 110 kDa
Observed molecular weight 150-180 kDa
GenBank accession numberBC010943
Gene Symbol OSMR
Gene ID (NCBI) 9180
RRIDAB_3672063
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ99650
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Oncostatin M (OSM) is a member of interleukin-6 family cytokines that modulates proliferation and differentiation in numerous cells (PMID: 22982441). OSM signals via a receptor complex comprising gp130 and the OSMR (also referred to as OSMRβ) (PMID: 8999038). OSMR is a single-pass membrane protein that belongs to the type I cytokine receptor family. OSMR mRNA is expressed relatively high in all neural cells and fibroblast, epithelial, and various tumor cell lines (PMID: 8999038). It has also been shown that OSMR associates with IL31RA to form the heterodimeric IL31 receptor (PMID: 15184896). The predicted molecular weight of OSMR is 110 kDa, and bands of 150-180 kDa can also be detected (PMID:8999038 ;34644107; 30226002; 9188471;22056139).

Protocols

Product Specific Protocols
WB protocol for OSMR antibody 84555-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols