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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

OSMR Recombinant antibody

OSMR Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

241865C8

Cat no : 84555-2-RR

Synonyms

Interleukin-31 receptor subunit beta, IL-31R-beta, IL-31RB, IL-31R subunit beta, IL-31 receptor subunit beta

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using OSMR Recombinant antibody (84555-2-RR)

    WB analysis using 84555-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84555-2-RR (OSMR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using OSMR Recombinant antibody (84555-2-RR)

    IHC staining of mouse cerebellum using 84555-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 84555-2-RR (OSMR antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHeLa cells, Raji cells, A375 cells, HEK-293 cells, HepG2 cells, SH-SY5Y cells, NIH/3T3 cells
Positive IHC detected inmouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84555-2-RR is OSMR Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen OSMR fusion protein Ag1426 相同性解析による交差性が予測される生物種
Full Name oncostatin M receptor
Calculated molecular weight 110 kDa
Observed molecular weight~180 kDa
GenBank accession numberBC010943
Gene symbol OSMR
Gene ID (NCBI) 9180
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Oncostatin M (OSM) is a member of interleukin-6 family cytokines that modulates proliferation and differentiation in numerous cells (PMID: 22982441). OSM signals via a receptor complex comprising gp130 and the OSMR (also called OSMRβ) (PMID: 8999038). OSMR is a single-pass membrane protein that belongs to the type I cytokine receptor family. OSMR mRNA is expressed at relatively high levels in all neural cells and fibroblast, epithelial, and various tumor cell lines (PMID: 8999038). It has also been shown that OSMR associates with IL31RA to form the heterodimeric IL31 receptor (PMID: 15184896). OMSR has a calculated molecular weight of 110 kDa, with an apparent molecular weight of 150-180 kDa (PMID: 8999038; 17028186; 39266541; 34644107). A 70 kDa soluble form can be detected by western blot (PMID: 17028186).

Protocols

Product Specific Protocols
WB protocol for OSMR antibody 84555-2-RRDownload protocol
IHC protocol for OSMR antibody 84555-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols