Validation Data Gallery
Tested Applications
Positive WB detected in | mouse embryo tissue, rat bladder tissue |
Positive IHC detected in | mouse embryo tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
30606-1-AP targets OSR1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | OSR1 fusion protein Ag31336 相同性解析による交差性が予測される生物種 |
Full Name | odd-skipped related 1 (Drosophila) |
Calculated molecular weight | 30 kDa |
Observed molecular weight | 30 kDa |
GenBank accession number | BC025712 |
Gene Symbol | OSR1 |
Gene ID (NCBI) | 130497 |
RRID | AB_3669737 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q8TAX0 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Odd-skipped-related1(OSR1), contains 3 C2H3-type zinc fingers, a tyrosine phosphorylation site and several putative PXXP SH3 binding motifs. It's a transcription factor that has a role in embryonic heart and urogenital development. Expressed during the beginning stages of embryogenesis and during limb and branchial arch development, it possibly participarts in the metanephric kidney formation. OSR1 also plays a key role in the generation of kidney precursor cells and their subsequent differentiation into nephrons. OSR1 is upregulated in several pancreatic and esopha-geal cancer cell lines and downregulated in some primary gastric cancers
Protocols
Product Specific Protocols | |
---|---|
WB protocol for OSR1 antibody 30606-1-AP | Download protocol |
IHC protocol for OSR1 antibody 30606-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |