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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using OSTA Polyclonal antibody (31330-1-AP)

    WB analysis using 31330-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31330-1-AP (SLC51A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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OSTA Polyclonal antibody

OSTA Polyclonal Antibody for WB, ELISA
Cat No. 31330-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Synonyms

SLC51A, OSTalpha, OST alpha

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse small intestine tissue, rat small intestine tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31330-1-AP targets OSTA in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen OSTA fusion protein Ag34517 相同性解析による交差性が予測される生物種
Full Name organic solute transporter alpha
Calculated molecular weight340 aa, 38 kDa
Observed molecular weight 38 kDa
GenBank accession numberNM_152672
Gene Symbol SLC51A
Gene ID (NCBI) 200931
RRIDAB_3669946
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ86UW1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SLC51A also known as OSTA, belongs to the OST-alpha family. SLC51A is an essential component of the Ost-alpha/Ost-beta complex, a heterodimer that acts as the intestinal basolateral transporter responsible for bile acid export from enterocytes into portal blood (PubMed:16317684). SLC51A is located in basolateral plasma membrane and transported from the endoplasmic reticulum to the plasma membrane upon interacting with SLC51B. SLC51A is expressed with a high expression in kidney and intestine (PMID: 15563450). Mutations in SLC51A are associated with Cholestasis progressive familial intrahepatic 6(PMID: 31863603).

Protocols

Product Specific Protocols
WB protocol for OSTA antibody 31330-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols