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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

OTC Monoclonal antibody, PBS Only

OTC Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat, pig

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2F11C7

Cat no : 67553-1-PBS

Synonyms

OCTD, ornithine carbamoyltransferase, Ornithine transcarbamylase, OTC, OTCase

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using OTC Monoclonal antibody (67553-1-Ig)

    WB analysis using 67553-1-Ig (same clone as 67553-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67553-1-Ig (OTC antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67553-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using OTC Monoclonal antibody (67553-1-Ig)

    IHC staining of human liver using 67553-1-Ig (same clone as 67553-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67553-1-Ig (OTC antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67553-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using OTC Monoclonal antibody (67553-1-Ig)

    IHC staining of human liver cancer using 67553-1-Ig (same clone as 67553-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67553-1-Ig (OTC antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67553-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat colon tissue using OTC Monoclonal antibody (67553-1-Ig)

    IHC staining of rat colon using 67553-1-Ig (same clone as 67553-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 67553-1-Ig (OTC antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67553-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67553-1-PBS targets OTC in WB, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Reactivity Human, mouse, rat, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen OTC fusion protein Ag24186 相同性解析による交差性が予測される生物種
Full Name ornithine carbamoyltransferase
Calculated molecular weight 354 aa, 40 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC074745
Gene symbol OTC
Gene ID (NCBI) 5009
RRIDAB_2882767
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Ornithine transcarbamylase (OTC) is a liver and intestinal mucosa-located, intramitochondrial, rate-limiting enzyme in the urea cycle. It catalyzes the reaction that converts ornithine and carbamoyl phosphate into citrulline, which is the second step of the urea cycle, to detoxify the ammonia produced from amino acid catabolism.