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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

OTOR Polyclonal antibody

OTOR Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 18132-1-AP

Synonyms

FDP, Fibrocyte derived protein, MIAL, MIAL1, OTOR, otoraplin

Validation Data Gallery

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  • Western Blot (WB) analysis of Transfected HEK-293 cells using OTOR Polyclonal antibody (18132-1-AP)

    WB analysis of Transfected HEK-293 using 18132-1-AP

    Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 18132-1-AP (OTOR Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using OTOR Polyclonal antibody (18132-1-AP)

    IHC staining of human liver cancer using 18132-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18132-1-AP (OTOR antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using OTOR Polyclonal antibody (18132-1-AP)

    IHC staining of human liver cancer using 18132-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18132-1-AP (OTOR antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using OTOR Polyclonal antibody (18132-1-AP)

    IHC staining of human ovary tumor using 18132-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 18132-1-AP (OTOR antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using OTOR Polyclonal antibody (18132-1-AP)

    IHC staining of human ovary tumor using 18132-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 18132-1-AP (OTOR antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive IHC detected inhuman liver cancer tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 18132-1-AP is OTOR Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen OTOR fusion protein Ag12714 相同性解析による交差性が予測される生物種
Full Name otoraplin
Calculated molecular weight 128 aa, 14 kDa
GenBank accession numberBC101688
Gene symbol OTOR
Gene ID (NCBI) 56914
RRIDAB_2878505
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for OTOR antibody 18132-1-APDownload protocol
IHC protocol for OTOR antibody 18132-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols