Validation Data Gallery

Filter: AllWBIHC

WB analysis of mouse colon using 29921-1-AP
mouse colon tissue were subjected to SDS PAGE followed by western blot with 29921-1-AP (OTUD1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis using 29921-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 29921-1-AP (OTUD1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis using 29921-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 29921-1-AP (OTUD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human cervical cancer using 29921-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 29921-1-AP (OTUD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse liver using 29921-1-AP
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 29921-1-AP (OTUD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human ovary tumor using 29921-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 29921-1-AP (OTUD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human ovary tumor using 29921-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 29921-1-AP (OTUD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human cervical cancer using 29921-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 29921-1-AP (OTUD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

OTUD1 Polyclonal antibody

OTUD1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 29921-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA, IF

Synonyms

OTU domain containing 1, OTDC1, DUBA7, DUBA 7

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Overview

Tested Applications
Product Information
Publications (2)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	mouse colon tissue, A549 cells
Positive IHC detected in	human cervical cancer tissue, human ovary tumor tissue, mouse liver tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 1 publications below
IF	See 1 publications below

Product Information

29921-1-AP targets OTUD1 in WB, IHC, ELISA, IF applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	OTUD1 fusion protein Ag31996 相同性解析による交差性が予測される生物種
Full Name	OTU domain containing 1
Calculated molecular weight	51 kDa
Observed molecular weight	50-55 kDa
Gene Symbol	OTUD1
Gene ID (NCBI)	220213
RRID	AB_3086190
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q5VV17
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

OTUD1, OTU domain-containing protein 1, is a deubiquitinating enzyme that specifically hydrolyzes 'Lys-63'-linked polyubiquitin to monoubiquitin (PMID: 23827681). OTUD1-mediated deubiquitination prevents activation of the ribosome quality control and subsequent dissociation of ribosomes and stimulates formation of polysomes and translation (PMID: 36445135). OTUD1 is a colon-specific deubiquitinase that expressed in normal colonic epithelium goblet cells (PMID: 36910614).

Protocols

Product Specific Protocols
WB protocol for OTUD1 antibody 29921-1-AP	Download protocol
IHC protocol for OTUD1 antibody 29921-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IF	Nat Commun Aggresome formation promotes ASK1/JNK signaling activation and stemness maintenance in ovarian cancer Authors - Yurou Chen View Article
		Int J Cardiol BECN1 regulates FADD/RIPK1/Caspase-8 complex formation via RIPK1 ubiquitination by downregulating OTUD1 in MI/R induced myocyte apoptosis Authors - Lu Hongquan View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of mouse colon using 29921-1-AP

WB analysis using 29921-1-AP