Validation Data Gallery
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Tested Applications
| Positive WB detected in | HEK-293T cells, HEK-293 cells, HeLa cells, Jurkat cells, K-562 cells, NIH/3T3 cells, C6 cells |
| Positive IF/ICC detected in | A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 8 publications below |
| IF | See 3 publications below |
| IP | See 2 publications below |
| CoIP | See 1 publications below |
Product Information
21002-1-AP targets OTUD5 in WB, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | OTUD5 fusion protein Ag15190 相同性解析による交差性が予測される生物種 |
| Full Name | OTU domain containing 5 |
| Calculated molecular weight | 571 aa, 61 kDa |
| Observed molecular weight | 70-75 kDa |
| GenBank accession number | BC028225 |
| Gene Symbol | OTUD5 |
| Gene ID (NCBI) | 55593 |
| RRID | AB_2878788 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96G74 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The deubiquitinating enzyme OTUD5, also known as deubiquitinating enzyme A (DUBA), is a member of the deubiquitinating enzyme OTU family. By deubiquitinating other proteins, OTUD5 is an important regulator of several physiological processes, such as immune signaling and DNA damage responses. The dysregulation of these processes can increase the risk of associated diseases, including inflammation, cancer and genetic disorders.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for OTUD5 antibody 21002-1-AP | Download protocol |
| IF protocol for OTUD5 antibody 21002-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun OTUD5 promotes end-joining of deprotected telomeres by promoting ATM-dependent phosphorylation of KAP1S824 | ||
Adv Sci (Weinh) OTUD5 Protects Dopaminergic Neurons by Promoting the Degradation of α-Synuclein in Parkinson's Disease Model | ||
Clin Transl Med p53 inhibits OTUD5 transcription to promote GPX4 degradation and induce ferroptosis in gastric cancer | ||
Int J Radiat Oncol Biol Phys USP11 exacerbates radiation induced pneumonitis by activating endothelial cell inflammatory response via OTUD5-STING signaling | ||
Carcinogenesis CacyBP promotes the development of lung adenocarcinoma by regulating OTUD5 | ||
Nat Commun Podocyte OTUD5 alleviates diabetic kidney disease through deubiquitinating TAK1 and reducing podocyte inflammation and injury |