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- KD/KO Validated
OTUD6B Polyclonal antibody
OTUD6B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
Cat no : 25430-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells, Jurkat cells, MCF-7 cells, PC-3 cells |
| Positive IHC detected in | human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | PC-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 1 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
Product Information
25430-1-AP targets OTUD6B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | OTUD6B fusion protein Ag22044 相同性解析による交差性が予測される生物種 |
| Full Name | OTU domain containing 6B |
| Calculated molecular weight | 293 aa, 34 kDa |
| Observed molecular weight | 34-40 kDa |
| GenBank accession number | BC029760 |
| Gene symbol | OTUD6B |
| Gene ID (NCBI) | 51633 |
| RRID | AB_2880075 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for OTUD6B antibody 25430-1-AP | Download protocol |
| IHC protocol for OTUD6B antibody 25430-1-AP | Download protocol |
| IF protocol for OTUD6B antibody 25430-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Mol Life Sci Otud6b induces pulmonary arterial hypertension by mediating the Calpain-1/HIF-1α signaling pathway
| ||
Cell Biol Int OTUD6B promotes cholangiocarcinoma growth by regulating STAT3 phosphorylation through deubiquitination of PTK2
|
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Dorota (Verified Customer) (04-11-2019) | Antibody works perfectly for western blotting, however I could not optimise it for immunofluorescence. I tried series of dilutions and couple of different blocking/fixing methods, but there was no difference between knockdown and control conditions in any of them.
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