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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

OVCA1 Polyclonal antibody

OVCA1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 20112-1-AP

Synonyms

DPH1, DPH2L1, DPH2L, DPH1 homolog, Diphtheria toxin resistance protein 1

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using OVCA1 Polyclonal antibody (20112-1-AP)

    WB analysis of HeLa using 20112-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 20112-1-AP (OVCA1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using OVCA1 Polyclonal antibody (20112-1-AP)

    IHC staining of human ovary cancer using 20112-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 20112-1-AP (OVCA1 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using OVCA1 Polyclonal antibody (20112-1-AP)

    IF Staining of HeLa using 20112-1-AP

    Immunofluorescent analysis of HeLa cells using 20112-1-AP (OVCA1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHeLa cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20112-1-AP targets OVCA1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen OVCA1 fusion protein Ag13910 相同性解析による交差性が予測される生物種
Full Name DPH1 homolog (S. cerevisiae)
Calculated molecular weight 489 aa, 52 kDa
Observed molecular weight 40-60 kDa
GenBank accession numberBC003099
Gene symbol DPH1
Gene ID (NCBI) 1801
RRIDAB_10641038
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

OVCA1, also named as DPH1, is an evolutionarily conserved gene, indicative of a fundamental biological role in a variety of species. In humans, OVCA1 was initially mapped within the deletion region of 17p13.3 involved in early ovarian cancer (PMID: 8564979). Ovca1-null mice, mainly addressed its tumor suppressive role and potential cooperative role in modifying p53-deficient tumorigenesis (PMID: 14744934). OVCA1 acts as a component of an enzyme complex with DPH2, DPH3 and DPH4 that functionally catalyzes the first step of the diphthamide biosynthesis pathway, to posttranslationally modify a conserved histidine residue (His699 in yeast and His715 in human) of eukaryotic elongation factor 2 (eEF-2).

Protocols

Product Specific Protocols
WB protocol for OVCA1 antibody 20112-1-APDownload protocol
IHC protocol for OVCA1 antibody 20112-1-APDownload protocol
IF protocol for OVCA1 antibody 20112-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols