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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using OVGP1 Polyclonal antibody (22324-1-AP)

    WB analysis using 22324-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22324-1-AP (OVGP1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse ovary tissue using OVGP1 Polyclonal antibody (22324-1-AP)

    IHC staining of mouse ovary using 22324-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 22324-1-AP (OVGP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

OVGP1 Polyclonal antibody

OVGP1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 22324-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

CHIT5, EGP, MUC9, Mucin 9, OGP, OVGP1, Oviduct specific glycoprotein, Oviductal glycoprotein, Oviductin

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman placenta tissue, mouse ovary tissue, mouse uterus tissue
Positive IHC detected inmouse ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22324-1-AP targets OVGP1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen OVGP1 fusion protein Ag17771 相同性解析による交差性が予測される生物種
Full Name oviductal glycoprotein 1, 120kDa
Calculated molecular weight 678 aa, 75 kDa
Observed molecular weight70 kDa
GenBank accession numberBC126177
Gene Symbol OVGP1
Gene ID (NCBI) 5016
RRIDAB_3085696
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ12889
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

OVGP1 (oviduct-specific glycoprotein, also known as MUC9) is a high molecular-weight glycoprotein thought to be exclusively secreted by the non-ciliated epithelial cells of the fallopian tube (PMID: 12444068). Identified first as an estrogen-induced secretory protein in the oviduct, OVGP1 aids in sperm capacitation, fertilization, and early embryonic development (PMID: 28883914). OVGP1 is the major non-serum protein present in OF (oviduct fluid) and its biological activity differs among species (PMID: 27601270).

Protocols

Product Specific Protocols
WB protocol for OVGP1 antibody 22324-1-APDownload protocol
IHC protocol for OVGP1 antibody 22324-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols