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Validation Data Gallery

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  • Western Blot (WB) analysis of HUVEC cells using OX40L/TNFSF4 Recombinant antibody (82794-7-RR)

    WB analysis of HUVEC using 82794-7-RR

    HUVEC cells were subjected to SDS PAGE followed by western blot with 82794-7-RR (OX40L/TNFSF4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of MJ cells using OX40L/TNFSF4 Recombinant antibody (82794-7-RR)

    IF Staining of MJ using 82794-7-RR

    Immunofluorescent analysis of (4% PFA) fixed MJ cells using OX40L/TNFSF4 antibody (82794-7-RR, Clone: 241813A6 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of MJ cells using OX40L/TNFSF4 Recombinant antibody (82794-7-RR)

    FC experiment of MJ using 82794-7-RR

    1x10^6 MJ cells were surface stained with 0.25 ug OX40L/TNFSF4 Recombinant antibody (82794-7-RR, Clone:241813A6) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were not fixed.

  • Affinity and Kinetic Characterization of 82794-7-RR

    Affinity and Kinetic Characterization of 82794-7-RR

    Biolayer interferometry (BLl) kinetic assays of 82794-7-RR against Human OX40L/TNFSF4 were performed. The affinity constant is 13.8 pM.

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OX40L/TNFSF4 Recombinant antibody

OX40L/TNFSF4 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 82794-7-RR
Clone No.241813A6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

OX40L, TNFSF4, TNFSF4/CD252, TNFSF 4, OX40 ligand

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHUVEC cells
Positive IF/ICC detected inMJ cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82794-7-RR targets OX40L/TNFSF4 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name tumor necrosis factor (ligand) superfamily, member 4
Observed molecular weight30 kDa
GenBank accession numberNM_003326.5
Gene Symbol OX40L/TNFSF4
Gene ID (NCBI) 7292
RRIDAB_3670552
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP23510
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for OX40L/TNFSF4 antibody 82794-7-RRDownload protocol
IF protocol for OX40L/TNFSF4 antibody 82794-7-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols