Validation Data Gallery
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Tested Applications
| Positive WB detected in | HepG2 cells, HeLa cells, NIH/3T3 cells, L02 cells, mouse liver tissue, rat liver tissue |
| Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 9 publications below |
| IF | See 1 publications below |
| IP | See 1 publications below |
Product Information
21055-1-AP targets OXA1L in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | OXA1L fusion protein Ag13819 相同性解析による交差性が予測される生物種 |
| Full Name | oxidase (cytochrome c) assembly 1-like |
| Calculated molecular weight | 437 aa, 49 kDa |
| Observed molecular weight | 42 kDa |
| GenBank accession number | BC001669 |
| Gene Symbol | OXA1L |
| Gene ID (NCBI) | 5018 |
| RRID | AB_10695769 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q15070 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
OXA1L/Oxa1 is a member of the conserved Oxa1/YidC/Alb3 protein family and is required for cytochrome c oxidase assembly. The OXA1L protein was initially identified in yeast proteins involved in the biogenesis of the mitochondrial respiratory chain complexes. It locates in the inner membrane of mitochondria and may be involved in mediating the insertion of proteins from the matrix into the inner membrane.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for OXA1L antibody 21055-1-AP | Download protocol |
| IHC protocol for OXA1L antibody 21055-1-AP | Download protocol |
| IF protocol for OXA1L antibody 21055-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Metab Proteolytic cleavage of opa1 stimulates mitochondrial inner membrane fusion and couples fusion to oxidative phosphorylation. | ||
Am J Hum Genet Recurrent De Novo Dominant Mutations in SLC25A4 Cause Severe Early-Onset Mitochondrial Disease and Loss of Mitochondrial DNA Copy Number. | ||
EMBO Mol Med OXA1L mutations cause mitochondrial encephalopathy and a combined oxidative phosphorylation defect.
| ||
Aging (Albany NY) Reduced mitochondrial translation prevents diet-induced metabolic dysfunction but not inflammation. | ||
J Cell Sci Cardiolipin is required for membrane docking of mitochondrial ribosomes and protein synthesis |