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  • Featured Product
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OXCT1 Monoclonal antibody

OXCT1 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1G1B9

Cat no : 67836-1-Ig

Synonyms

SCOT, OXCT, EC:2.8.3.5, 3-oxoacid CoA-transferase 1, 3 oxoacid CoA transferase 1

Validation Data Gallery

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  • Western Blot (WB) analysis of rat heart tissue using OXCT1 Monoclonal antibody (67836-1-Ig)

    WB analysis of rat heart using 67836-1-Ig

    rat heart tissue were subjected to SDS PAGE followed by western blot with 67836-1-Ig (SCOT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using OXCT1 Monoclonal antibody (67836-1-Ig)

    WB analysis of HeLa using 67836-1-Ig

    WB result of OXCT1 antibody (67836-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-OXCT1 transfected HeLa cells.

  • Western Blot (WB) analysis of mouse brain tissue using OXCT1 Monoclonal antibody (67836-1-Ig)

    WB analysis of mouse brain using 67836-1-Ig

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 67836-1-Ig (SCOT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rabbit brain tissue using OXCT1 Monoclonal antibody (67836-1-Ig)

    WB analysis of rabbit brain using 67836-1-Ig

    rabbit brain tissue were subjected to SDS PAGE followed by western blot with 67836-1-Ig (SCOT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using OXCT1 Monoclonal antibody (67836-1-Ig)

    WB analysis of HeLa using 67836-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 67836-1-Ig (SCOT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using OXCT1 Monoclonal antibody (67836-1-Ig)

    WB analysis of Jurkat using 67836-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67836-1-Ig (SCOT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig brain tissue using OXCT1 Monoclonal antibody (67836-1-Ig)

    WB analysis of pig brain using 67836-1-Ig

    pig brain tissue were subjected to SDS PAGE followed by western blot with 67836-1-Ig (SCOT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using OXCT1 Monoclonal antibody (67836-1-Ig)

    WB analysis of rat brain using 67836-1-Ig

    rat brain tissue were subjected to SDS PAGE followed by western blot with 67836-1-Ig (SCOT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of rat brain tissue using OXCT1 Monoclonal antibody (67836-1-Ig)

    IHC staining of rat brain using 67836-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67836-1-Ig (OXCT1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using OXCT1 Monoclonal antibody (67836-1-Ig)

    IF Staining of MCF-7 using 67836-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using OXCT1 antibody (67836-1-Ig, Clone: 1G1B9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using OXCT1 Monoclonal antibody (67836-1-Ig)

    IF Staining of HeLa using 67836-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using OXCT1 antibody (67836-1-Ig, Clone: 1G1B9 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using OXCT1 Monoclonal antibody (67836-1-Ig)

    IF Staining of HeLa using 67836-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using OXCT1 antibody (67836-1-Ig, Clone: 1G1B9 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HeLa cells using OXCT1 Monoclonal antibody (67836-1-Ig)

    FC experiment of HeLa using 67836-1-Ig

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human OXCT1 (67836-1-Ig, Clone:1G1B9) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inrat heart tissue, HeLa cells, Jurkat cells, pig brain tissue, rat brain tissue, mouse brain tissue, rabbit brain tissue
Positive IHC detected inrat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells, HeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

67836-1-Ig targets OXCT1 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Reactivity human, mouse, rat, pig, rabbit
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen OXCT1 fusion protein Ag24792 相同性解析による交差性が予測される生物種
Full Name 3-oxoacid CoA transferase 1
Calculated molecular weight 520 aa, 56 kDa
Observed molecular weight52-56 kDa
GenBank accession numberBC009001
Gene symbol OXCT1
Gene ID (NCBI) 5019
RRIDAB_2918598
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

OXCT1 (also know as SCOT), encoded by nuclear gene, is a mitochondrial CoA transferase required for ketone body degradation. It catalyzes the transfer of CoA from succinyl-CoA to acetoacetate, generating acetoacetyl-CoA. OXCT1 is expressed in brain, heart, and skeletal muscle, but not in liver. This antibody specifically recognizes endogenous OXCT1. (21209089)

Protocols

Product Specific Protocols
WB protocol for OXCT1 antibody 67836-1-IgDownload protocol
IHC protocol for OXCT1 antibody 67836-1-IgDownload protocol
IF protocol for OXCT1 antibody 67836-1-IgDownload protocol
FC protocol for OXCT1 antibody 67836-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancer Med

Induction of resistance to neurotrophic tropomyosin-receptor kinase inhibitors by HMGCS2 via a mevalonate pathway

Authors - Yasuhiro Kato
View Article