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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

OXER1 Polyclonal antibody

OXER1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 55202-1-AP

Synonyms

G-protein coupled receptor R527, G-protein coupled receptor 170, GPR170, GPCR, G protein coupled receptor 170

Validation Data Gallery

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  • Western Blot (WB) analysis of K-562 cells using OXER1 Polyclonal antibody (55202-1-AP)

    WB analysis of K-562 using 55202-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 55202-1-AP (OXER1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using OXER1 Polyclonal antibody (55202-1-AP)

    IHC staining of human kidney using 55202-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 55202-1-AP (OXER1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using OXER1 Polyclonal antibody (55202-1-AP)

    IHC staining of human kidney using 55202-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 55202-1-AP (OXER1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using OXER1 Polyclonal antibody (55202-1-AP)

    IHC staining of human liver using 55202-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 55202-1-AP (OXER1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using OXER1 Polyclonal antibody (55202-1-AP)

    IHC staining of human liver using 55202-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 55202-1-AP (OXER1 antibody) at dilution of 1:50 (under 40x lens).

  • Flow cytometry (FC) experiment of K-562 cells using OXER1 Polyclonal antibody (55202-1-AP)

    FC experiment of K-562 using 55202-1-AP

    1x10^6 K-562 cells were intracellularly stained with 0.4 ug OXER1 Polyclonal antibody (55202-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inK-562 cells
Positive IHC detected inhuman kidney tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inK-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

55202-1-AP targets OXER1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name oxoeicosanoid (OXE) receptor 1
Calculated molecular weight 46 kDa
Observed molecular weight 43-46 kDa
GenBank accession numberNM_148962
Gene symbol OXER1
Gene ID (NCBI) 165140
RRIDAB_10858396
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

OXER1, also named as GPR170 and TG1019, is a receptor for eicosanoids and polyunsaturated fatty acids such as 5-oxo-6E,8Z,11Z,14Z-eicosatetraenoic acid (5-OXO-ETE), 5(S)-hydroperoxy-6E,8Z,11Z,14Z-eicosatetraenoic acid (5(S)-HPETE) and arachidonic acid. It seems to be coupled to the G(i)/G(o), families of heteromeric G proteins. (PMID:12065583) This antibody is specific to OXER1.

Protocols

Product Specific Protocols
WB protocol for OXER1 antibody 55202-1-APDownload protocol
IHC protocol for OXER1 antibody 55202-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cell Endocrinol

Nuclear translocation of the membrane oxoeicosanoid/androgen receptor, OXER1: possible mechanisms involved

Authors - Panagiotis Malamos
View Article