OXPHOS Cocktail (Human recommended)
Cat no : PK30006
Validation Data Gallery
Product Information
OXPHOS (oxidative phosphorylation) requires the transport of electrons to molecular oxygen by the mitochondrial respiratory chain which involves four multi-subunit complexes known as complexes I, II, III, and IV (CI, CII, CIII and CIV) and two mobile electron carriers, coenzyme Q (CoQ) and cytochrome c. The respiratory chain generates a transmembrane proton gradient that is channeled by complex V (also known as ATP synthase, CV) to synthesize ATP.
This OXPHOS kit contains five Polyclonal antibodies. The kit is suitable for Western Blotting analysis of the relative levels of the 5 OXPHOS complexes in mitochondrial preparations from human. Individual proteins that need to be tested can be purchased according to the corresponding catalog number.
| Cat.No | Name | MW | Size | ||
| Antibodies | 14676-1-AP | ATP5A1 | Complexes Ⅴ | 50-55 kDa | 150ul |
| 21705-1-AP | UQCRC1 | Complexes Ⅲ | 45 kDa | ||
| 10620-1-AP | SDHB | Complexes Ⅱ | 32 kDa | ||
| 55070-1-AP | MTCO2 | Complexes Ⅳ | 23-25 kDa | ||
| 14794-1-AP | NDUFB8 | ComplexesⅠ | 19-22 kDa |
Storage
Store at -20℃. Stable for one year after shipment.
Usage
1.Refer to the western blot protocol. (https://www.ptglab.com/support/western-blot-protocol/western-blot-protocol/)