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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

OXT Polyclonal antibody

OXT Polyclonal Antibody for IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 18041-1-AP

Synonyms

Oxytocin-neurophysin 1, Oxytocin neurophysin 1, Oxytocin, Ocytocin Neurophysin 1, Neurophysin 1

Validation Data Gallery

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  • Immunoprecipitation (IP) experiment of mouse pituitary gland tissue using OXT Polyclonal antibody (18041-1-AP)

    IP experiment of mouse pituitary gland using 18041-1-AP

    IP result of anti-OXT (IP:18041-1-AP, 4ug; Detection:18041-1-AP 1:300) with mouse pituitary gland tissue lysate 1440 ug.

  • Immunohistochemistry (IHC) staining of human pituitary tissue using OXT Polyclonal antibody (18041-1-AP)

    IHC staining of human pituitary using 18041-1-AP

    Immunohistochemical analysis of paraffin-embedded human pituitary tissue slide using 18041-1-AP (OXT antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pituitary tissue using OXT Polyclonal antibody (18041-1-AP)

    IHC staining of human pituitary using 18041-1-AP

    Immunohistochemical analysis of paraffin-embedded human pituitary tissue slide using 18041-1-AP (OXT antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using OXT Polyclonal antibody (18041-1-AP)

    IHC staining of mouse brain using 18041-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18041-1-AP (OXT antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse ovary tissue using OXT Polyclonal antibody (18041-1-AP)

    IHC staining of mouse ovary using 18041-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 18041-1-AP (OXT antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse ovary tissue using OXT Polyclonal antibody (18041-1-AP)

    IHC staining of mouse ovary using 18041-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 18041-1-AP (OXT antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive IP detected inmouse pituitary gland tissue
Positive IHC detected inhuman pituitary tissue, mouse ovary tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18041-1-AP targets OXT in IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen OXT fusion protein Ag12672 相同性解析による交差性が予測される生物種
Full Name oxytocin, prepropeptide
Calculated molecular weight 125 aa, 13 kDa
Observed molecular weight13 kDa
GenBank accession numberBC101841
Gene symbol OXT
Gene ID (NCBI) 5020
RRIDAB_2918056
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IHC protocol for OXT antibody 18041-1-APDownload protocol
IP protocol for OXT antibody 18041-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols