Validation Data Gallery

Filter: AllWBIPIHCIF-FroIF/ICC

WB analysis using 27260-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 27260-1-AP (Occludin antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours.
WB analysis using 27260-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 27260-1-AP (Occludin antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours.
WB analysis using 27260-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 27260-1-AP (Occludin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis using 27260-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 27260-1-AP (Occludin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis using 27260-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 27260-1-AP (Occludin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
IP experiment of HUVEC using 27260-1-AP
IP result of anti-Occludin (IP:27260-1-AP, 4ug; Detection:27260-1-AP 1:300) with HUVEC cells lysate 3600 ug.
IHC staining of human kidney using 27260-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27260-1-AP (Occludin antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human kidney using 27260-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27260-1-AP (Occludin antibody) at dilution of 1:200 (under 40x lens).
IHC staining of human colon using 27260-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27260-1-AP (Occludin antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon using 27260-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27260-1-AP (Occludin antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 27260-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27260-1-AP (Occludin Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 27260-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27260-1-AP (Occludin Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse brain using 27260-1-AP
Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using Occludin antibody (27260-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
IF Staining of Caco-2 using 27260-1-AP
Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using Occludin antibody (27260-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).
IF Staining of Caco-2 using 27260-1-AP
Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using Occludin antibody (27260-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of MCF-7 using 27260-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using Occludin antibody (27260-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Occludin Polyclonal antibody

Occludin Polyclonal Antibody for WB, IHC, IF/ICC, IF-Fro, IP, ELISA

Cat No. 27260-1-AP

Publications(593)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (7)

Applications

WB, IHC, IF/ICC, IF-Fro, IP, ELISA

Synonyms

OCLN

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite®594

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (593)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HUVEC cells, HepG2 cells, mouse kidney tissue, mouse liver tissue, rat liver tissue, mouse brain tissue, mouse colon tissue, rat brain tissue
Positive IP detected in	HUVEC cells
Positive IHC detected in	human colon tissue, human breast cancer tissue, human kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-Fro detected in	mouse brain tissue
Positive IF/ICC detected in	Caco-2 cells, MCF-7 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:1000-1:10000
Immunofluorescence (IF)-FRO	IF-FRO : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 467 publications below
IHC	See 86 publications below
IF	See 170 publications below
IP	See 1 publications below
FC	See 1 publications below

Product Information

27260-1-AP targets Occludin in WB, IHC, IF/ICC, IF-Fro, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig, rabbit, canine, chicken, bovine, goat, duck
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Occludin fusion protein Ag26173 相同性解析による交差性が予測される生物種
Full Name	occludin
Calculated molecular weight	522 aa, 59 kDa
Observed molecular weight	59 kDa, 32 kDa, 23-25 kDa
GenBank accession number	BC029886
Gene Symbol	Occludin
Gene ID (NCBI)	4950
RRID	AB_2880820
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q16625
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Occludin is an integral membrane protein located at the tight junction. It is a tetraspanin protein with four transmembrane domains, intracellular N and C termini, and two extracellular loops. Occludin plays a role in the formation and regulation of the tight junction paracellular permeability barrier. Occludin can exist in different isoforms, owing to modifications at the posttranscriptional and posttranslational levels, the monomeric occludin migrates as 53-65 kDa on SDS-PAGE (PMID: 22083955; 19457074).

Protocols

Product Specific Protocols
WB protocol for Occludin antibody 27260-1-AP	Download protocol
IHC protocol for Occludin antibody 27260-1-AP	Download protocol
IF protocol for Occludin antibody 27260-1-AP	Download protocol
IP protocol for Occludin antibody 27260-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	IHC	Cell Metab Acetate enables metabolic fitness and cognitive performance during sleep disruption Authors - Qinqin He View Article
mouse	IF	J Clin Invest A20 regulates lymphocyte adhesion in murine neuroinflammation by restricting endothelial ICOSL expression in the CNS Authors - Lisa Johann View Article
pig	WB	Microbiome Zearalenone disturbs the reproductive-immune axis in pigs: the role of gut microbial metabolites Authors - Shujin Wang View Article
	IF	Microbiome Arula-7 powder improves diarrhea and intestinal epithelial tight junction function associated with its regulation of intestinal flora in calves infected with pathogenic Escherichia coli O1 Authors - Hao Chen View Article
mouse	IF	Adv Sci (Weinh) Plasma-Activated Solutions Mitigates DSS-Induced Colitis via Restoring Redox Homeostasis and Reversing Microbiota Dysbiosis Authors - Tuanhe Sun View Article
human	WB	J Extracell Vesicles Extracellular vesicle-mediated delivery of circDYM alleviates CUS-induced depressive-like behaviours. Authors - Xiaoyu Yu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 27260-1-AP

WB analysis using 27260-1-AP

WB analysis using 27260-1-AP

WB analysis using 27260-1-AP

WB analysis using 27260-1-AP

IP experiment of HUVEC using 27260-1-AP

IHC staining of human kidney using 27260-1-AP

IHC staining of human kidney using 27260-1-AP

IHC staining of human colon using 27260-1-AP

IHC staining of human colon using 27260-1-AP

IHC staining of human breast cancer using 27260-1-AP

IHC staining of human breast cancer using 27260-1-AP

IF Staining of mouse brain using 27260-1-AP

IF Staining of Caco-2 using 27260-1-AP

IF Staining of Caco-2 using 27260-1-AP

IF Staining of MCF-7 using 27260-1-AP

Occludin Polyclonal antibody

Occludin Polyclonal Antibody for WB, IHC, IF/ICC, IF-Fro, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Acetate enables metabolic fitness and cognitive performance during sleep disruption

A20 regulates lymphocyte adhesion in murine neuroinflammation by restricting endothelial ICOSL expression in the CNS

Zearalenone disturbs the reproductive-immune axis in pigs: the role of gut microbial metabolites

Arula-7 powder improves diarrhea and intestinal epithelial tight junction function associated with its regulation of intestinal flora in calves infected with pathogenic Escherichia coli O1

Plasma-Activated Solutions Mitigates DSS-Induced Colitis via Restoring Redox Homeostasis and Reversing Microbiota Dysbiosis

Extracellular vesicle-mediated delivery of circDYM alleviates CUS-induced depressive-like behaviours.