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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Occludin Polyclonal antibody

Occludin Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (6)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 27260-1-AP

Synonyms

OCLN

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using Occludin Polyclonal antibody (27260-1-AP)

    WB analysis using 27260-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27260-1-AP (Occludin antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using Occludin Polyclonal antibody (27260-1-AP)

    WB analysis using 27260-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27260-1-AP (Occludin antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using Occludin Polyclonal antibody (27260-1-AP)

    WB analysis using 27260-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27260-1-AP (Occludin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using Occludin Polyclonal antibody (27260-1-AP)

    WB analysis using 27260-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27260-1-AP (Occludin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using Occludin Polyclonal antibody (27260-1-AP)

    WB analysis using 27260-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27260-1-AP (Occludin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HUVEC cells using Occludin Polyclonal antibody (27260-1-AP)

    IP experiment of HUVEC using 27260-1-AP

    IP result of anti-Occludin (IP:27260-1-AP, 4ug; Detection:27260-1-AP 1:300) with HUVEC cells lysate 3600 ug.

  • Immunohistochemistry (IHC) staining of human kidney tissue using Occludin Polyclonal antibody (27260-1-AP)

    IHC staining of human kidney using 27260-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27260-1-AP (Occludin antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using Occludin Polyclonal antibody (27260-1-AP)

    IHC staining of human kidney using 27260-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27260-1-AP (Occludin antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using Occludin Polyclonal antibody (27260-1-AP)

    IHC staining of human colon using 27260-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27260-1-AP (Occludin antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using Occludin Polyclonal antibody (27260-1-AP)

    IHC staining of human colon using 27260-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27260-1-AP (Occludin antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Occludin Polyclonal antibody (27260-1-AP)

    IHC staining of human breast cancer using 27260-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27260-1-AP (Occludin Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Occludin Polyclonal antibody (27260-1-AP)

    IHC staining of human breast cancer using 27260-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27260-1-AP (Occludin Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of Caco-2 cells using Occludin Polyclonal antibody (27260-1-AP)

    IF Staining of Caco-2 using 27260-1-AP

    Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using Occludin antibody (27260-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

  • Immunofluorescence (IF) / fluorescent staining of Caco-2 cells using Occludin Polyclonal antibody (27260-1-AP)

    IF Staining of Caco-2 using 27260-1-AP

    Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using Occludin antibody (27260-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using Occludin Polyclonal antibody (27260-1-AP)

    IF Staining of MCF-7 using 27260-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using Occludin antibody (27260-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHUVEC cells, HepG2 cells, mouse kidney tissue, mouse liver tissue, rat liver tissue, mouse brain tissue, mouse colon tissue, rat brain tissue
Positive IP detected inHUVEC cells
Positive IHC detected inhuman colon tissue, human breast cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inCaco-2 cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:1000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27260-1-AP targets Occludin in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig, rabbit, canine, bovine, goat, duck
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Occludin fusion protein Ag26173 相同性解析による交差性が予測される生物種
Full Name occludin
Calculated molecular weight 522 aa, 59 kDa
Observed molecular weight 59 kDa, 32 kDa, 23-25 kDa
GenBank accession numberBC029886
Gene symbol Occludin
Gene ID (NCBI) 4950
RRIDAB_2880820
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Occludin is an integral membrane protein located at the tight junction. It is a tetraspanin protein with four transmembrane domains, intracellular N and C termini and two extracellular loops. Occludin plays a role in the formation and regulation of the tight junction paracellular permeability barrier. Occludin can exist in different isoforms, owing to modifications at the posttranscriptional and posttranslational levels, the monomeric occludin migrates as 53-65 kDa on SDS-PAGE (PMID: 22083955; 19457074).

Protocols

Product Specific Protocols
WB protocol for Occludin antibody 27260-1-APDownload protocol
IHC protocol for Occludin antibody 27260-1-APDownload protocol
IF protocol for Occludin antibody 27260-1-APDownload protocol
IP protocol for Occludin antibody 27260-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

Cell Metab

Acetate enables metabolic fitness and cognitive performance during sleep disruption

Authors - Qinqin He
View Article
mouseIF

J Clin Invest

A20 regulates lymphocyte adhesion in murine neuroinflammation by restricting endothelial ICOSL expression in the CNS

Authors - Lisa Johann
View Article
IF

Microbiome

Arula-7 powder improves diarrhea and intestinal epithelial tight junction function associated with its regulation of intestinal flora in calves infected with pathogenic Escherichia coli O1

Authors - Hao Chen
View Article
pigWB

Microbiome

Zearalenone disturbs the reproductive-immune axis in pigs: the role of gut microbial metabolites

Authors - Shujin Wang
View Article
mouseIF

Adv Sci (Weinh)

Plasma-Activated Solutions Mitigates DSS-Induced Colitis via Restoring Redox Homeostasis and Reversing Microbiota Dysbiosis

Authors - Tuanhe Sun
View Article
humanWB

J Extracell Vesicles

Extracellular vesicle-mediated delivery of circDYM alleviates CUS-induced depressive-like behaviours.

Authors - Xiaoyu Yu
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Iram (Verified Customer) (06-13-2022)

Excellent antibody for immunoblotting

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: colon cancer cells
FH

Balawant (Verified Customer) (05-09-2022)

This antibody I have used and is working great . 1:2000 dilution is working great for the cell line (Colonic epithelialcellr of human).

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: Human colonic epithelial cells and mice colon tissue
FH

Kushal (Verified Customer) (03-14-2022)

  • Applications: Western Blot, Immunoprecipitation, ChIP
  • Primary Antibody Dilution: 1.2000
  • Cell Tissue Type: Surgical human tissue, mouse tissue, Caco-2 cells