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Osteopontin Polyclonal antibody

Osteopontin Polyclonal Antibody for WB, IHC, ELISA
Cat No. 25715-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

SPP1, PSEC0156, OPN, ETA 1, BSPI

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size/Concentration: 

-/ -


ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inmouse kidney tissue, HEK-293, rat kidney tissue, HEK-293 cells, C2C12 cells
Positive IHC detected inhuman stomach cancer tissue, human kidney tissue, human small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25715-1-AP targets Osteopontin in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Osteopontin fusion protein Ag22588 相同性解析による交差性が予測される生物種
Full Name secreted phosphoprotein 1
Calculated molecular weight 314 aa, 35 kDa
Observed molecular weight 66 kDa
GenBank accession numberBC007016
Gene Symbol Osteopontin
Gene ID (NCBI) 6696
RRIDAB_2880206
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP10451
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Osteopontin (OPN), also known as SPP1, is a secreted glycophosphoprotein that belongs to the small integrin-binding ligand N-linked glycoprotein (SIBLING) family. Originally isolated from bone, OPN has been found in kidney, vascular tissues, biological fluids, and various tumor tissues (PMID: 15138464; 16406521). OPN can interact with CD44 and integrins and regulate diverse biological processes. It has a multifaceted role in bone development and remodeling, and is also involved in the inflammatory and immune response, oncogenesis and cancer progression. The very acidic nature of OPN, as well as the presence of variable posttranslational modifications, has led to anomalous migration in SDS-polyacrylamide gels and therefore to reports of different molecular weights for OPN (PMID: 8293561). Depending on the cell and tissue source and/or the SDS-PAGE system, OPN migrates with a molecular weight of 44-80 kDa, as well as at some smaller bands correspond to peptide fragments (PMID: 8195113; 17890765).

Protocols

Product Specific Protocols
WB protocol for Osteopontin antibody 25715-1-APDownload protocol
IHC protocol for Osteopontin antibody 25715-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

Nat Neurosci

Microglia and macrophages promote corralling, wound compaction and recovery after spinal cord injury via Plexin-B2.

Authors - Xiang Zhou
mouseIHC

Sci Adv

Diversified transcriptional responses of myeloid and glial cells in spinal cord injury shaped by HDAC3 activity.

Authors - Shalaka Wahane
mouseWB

ACS Appl Mater Interfaces

ZIF-8-Modified Black Phosphorus Nanosheets Incorporated into Injectable Dual-Component Hydrogels for Enhanced Photothermal Antibacterial and Osteogenic Activities

Authors - Quan Li
mouseIHC

Part Fibre Toxicol

Aryl hydrocarbon receptor activation-mediated vascular toxicity of ambient fine particulate matter: contribution of polycyclic aromatic hydrocarbons and osteopontin as a biomarker.

Authors - Chia-Chi Ho
humanIF

J Transl Med

Multi-omics-driven discovery of invasive patterns and treatment strategies in CA19-9 positive intrahepatic cholangiocarcinoma

Authors - Delin Ma
mouseWB

Cell Mol Life Sci

Metastasis suppressor 1 controls osteoblast differentiation and bone homeostasis through regulating Src-Wnt/β-catenin signaling.

Authors - Meng Chen
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