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Host
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Mouse Osteopontin Recombinant antibody, PBS Only

Osteopontin Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240250A1

Cat no : 83413-6-PBS

Synonyms

Spp1, Opn, 240250A1

Validation Data Gallery

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  • Western Blot (WB) analysis of RAW 264.7 cells using Osteopontin Recombinant antibody (83413-6-RR)

    WB analysis of RAW 264.7 using 83413-6-RR (same clone as 83413-6-PBS)

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 83413-6-RR (Osteopontin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83413-6-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of RAW 264.7 cells using Osteopontin Recombinant antibody (83413-6-RR)

    IF Staining of RAW 264.7 using 83413-6-RR (same clone as 83413-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed LPS and Brefeldin A treated RAW 264.7 cells using Osteopontin antibody (83413-6-RR, Clone: 240250A1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83413-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83413-6-RR

    Affinity and Kinetic Characterization of 83413-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83413-6-RR against Mouse Osteopontin were performed. The affinity constant is 2.19 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 83413-6-PBS is Osteopontin Fusion Protein expressed in E. coli.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name secreted phosphoprotein 1
Calculated molecular weight32kd
GenBank accession numberNM_001204233.1
Gene symbol Osteopontin
Gene ID (NCBI) 20750
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.