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Validation Data Gallery

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  • Western Blot (WB) analysis of RAW 264.7 cells using Osteopontin Recombinant antibody (83413-6-RR)

    WB analysis of RAW 264.7 using 83413-6-RR (same clone as 83413-6-PBS)

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 83413-6-RR (Osteopontin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83413-6-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse stomach tissue using Osteopontin Recombinant antibody (83413-6-RR)

    IF Staining of mouse stomach using 83413-6-RR (same clone as 83413-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse stomach tissue using Osteopontin antibody (83413-6-RR, Clone: 240250A1 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83413-6-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of RAW 264.7 cells using Osteopontin Recombinant antibody (83413-6-RR)

    IF Staining of RAW 264.7 using 83413-6-RR (same clone as 83413-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed LPS and Brefeldin A treated RAW 264.7 cells using Osteopontin antibody (83413-6-RR, Clone: 240250A1 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83413-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83413-6-RR

    Affinity and Kinetic Characterization of 83413-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83413-6-RR against Mouse Osteopontin were performed. The affinity constant is 2.19 nM.

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Mouse Osteopontin Recombinant antibody, PBS Only

Osteopontin Uni-rAbTM Recombinant Antibody for WB, IF/ICC, IF-P, ELISA
Cat No. 83413-6-PBS
Clone No.240250A1

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IF/ICC, IF-P, ELISA

Synonyms

Spp1, Opn, 240250A1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83413-6-PBS targets Osteopontin in WB, IF/ICC, IF-P, ELISA applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name secreted phosphoprotein 1
Calculated molecular weight32kd
Observed molecular weight 60 kDa
GenBank accession numberNM_001204233.1
Gene Symbol Osteopontin
Gene ID (NCBI) 20750
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ547B5
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Osteopontin (OPN) is also named as 2AR, minopontin, bone sialoprotein 1, Spp1, Eta-1 and Op, and belongs to the osteopontin family. Osteopontin is major non-collagenous bone protein that binds tightly to hydroxyapatite. It is a highly phosphorylated glycophosphoprotein having acidic characteristics and rich in aspartic acid (PMID: 30003880). And it acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity (PMID:2351930, PMID:10657301). OPN, a multifunctional protein, has important functions on cardiovascular diseases, cancer, diabetes and kidney stone diseases and in the process of inflammation, biomineralization, cell viability and wound healing (PMID: 30003880). Osteopontin (OPN) is a pleiotropic glycoprotein expressed in various cell types in animals and in humans, including bone, immune, smooth muscle, epithelial and endothelial cells. Moreover, OPN is found in kidneys (in the thick ascending limbs of the loop of Henle and in distal nephrons) and urine (PMID:30456594).