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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Osteoprotegerin/TNFRSF11B Polyclonal antibody (31766-1-AP)

    WB analysis using 31766-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31766-1-AP (Osteoprotegerin/TNFRSF11B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of rat kidney tissue using Osteoprotegerin/TNFRSF11B Polyclonal antibody (31766-1-AP)

    IHC staining of rat kidney using 31766-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 31766-1-AP (Osteoprotegerin/TNFRSF11B antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat kidney tissue using Osteoprotegerin/TNFRSF11B Polyclonal antibody (31766-1-AP)

    IHC staining of rat kidney using 31766-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 31766-1-AP (Osteoprotegerin/TNFRSF11B antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Osteoprotegerin/TNFRSF11B Polyclonal antibody (31766-1-AP)

    IF Staining of HepG2 using 31766-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using Osteoprotegerin/TNFRSF11B antibody (31766-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

Osteoprotegerin/TNFRSF11B Polyclonal antibody

Osteoprotegerin/TNFRSF11B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 31766-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

OCIF, OPG, Osteoclastogenesis inhibitory factor, Osteoprotegerin, TNFRSF11B

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inrat kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31766-1-AP targets Osteoprotegerin/TNFRSF11B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Osteoprotegerin/TNFRSF11B fusion protein Eg0976 相同性解析による交差性が予測される生物種
Full Name tumor necrosis factor receptor superfamily, member 11b
Calculated molecular weight46kDa
Observed molecular weight56-60 kDa
GenBank accession numberNM_002546.4
Gene Symbol TNFRSF11B
Gene ID (NCBI) 4982
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDO00300
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Osteoprotegerin (OPG), also known as tumor necrosis factor receptor superfamily member 11B (TNFRSF11B) and osteoclastogenesis inhibitory factor (OCIF), belongs to the tumor necrosis factor (TNF) receptor superfamily. By binding to RANKL, OPG inhibits osteoclastic activity and promotes bone formations. OPG plays a key role on cell survival by inhibiting TRAIL-induced apoptosis. OPG also is involved in vascular biology, fibrosis, immunity, EMT, and the apoptosis of cancer cells (PMID: 35523775).

Protocols

Product Specific Protocols
WB protocol for Osteoprotegerin/TNFRSF11B antibody 31766-1-APDownload protocol
IHC protocol for Osteoprotegerin/TNFRSF11B antibody 31766-1-APDownload protocol
IF protocol for Osteoprotegerin/TNFRSF11B antibody 31766-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols