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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

P-cadherin Monoclonal antibody, PBS Only

P-cadherin Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1G10F7

Cat no : 66295-1-PBS

Synonyms

Cadherin 3, CDH3, CDHP, HJMD, P cadherin, PCAD, P-cadherin, Placental cadherin

Validation Data Gallery

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  • Western Blot (WB) analysis of A431 cells using P-cadherin Monoclonal antibody (66295-1-Ig)

    WB analysis of A431 using 66295-1-Ig (same clone as 66295-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 66295-1-Ig (P-cadherin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66295-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using P-cadherin Monoclonal antibody (66295-1-Ig)

    IHC staining of human placenta using 66295-1-Ig (same clone as 66295-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66295-1-Ig (P-cadherin antibody) at dilution of 1:250 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66295-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using P-cadherin Monoclonal antibody (66295-1-Ig)

    IHC staining of human placenta using 66295-1-Ig (same clone as 66295-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66295-1-Ig (P-cadherin antibody) at dilution of 1:250 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66295-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66295-1-PBS targets P-cadherin in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen P-cadherin fusion protein Ag4758 相同性解析による交差性が予測される生物種
Full Name cadherin 3, type 1, P-cadherin (placental)
Calculated molecular weight 829 aa, 91 kDa
Observed molecular weight 120 kDa
GenBank accession numberBC041846
Gene symbol CDH3
Gene ID (NCBI) 1001
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. P-cadherin (placental cadherin), also known as CDH3 (cadherin 3), is a classical member of the cadherin superfamily which also include E-, N-, R-, and B-cadherins. Aberrant expression of P-cadherin has been reported in many cancers, including breast, gastric, endometrial, ovarian, and prostate cancer.