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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

P-cadherin Monoclonal antibody, PBS Only

P-cadherin Monoclonal Antibody for WB, FC, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, FC, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1G10F7

Cat no : 66295-1-PBS

Synonyms

Cadherin 3, CDH3, CDHP, HJMD, P cadherin, PCAD, P-cadherin, Placental cadherin

Validation Data Gallery

Applications:
  • WB analysis of A431 using 66295-1-Ig (same clone as 66295-1-PBS)

    WB analysis of A431 using 66295-1-Ig (same clone as 66295-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 66295-1-Ig (P-cadherin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66295-1-PBS in a different storage buffer formulation.

  • IHC staining of human placenta using 66295-1-Ig (same clone as 66295-1-PBS)

    IHC staining of human placenta using 66295-1-Ig (same clone as 66295-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66295-1-Ig (P-cadherin antibody) at dilution of 1:250 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66295-1-PBS in a different storage buffer formulation.

  • IHC staining of human placenta using 66295-1-Ig (same clone as 66295-1-PBS)

    IHC staining of human placenta using 66295-1-Ig (same clone as 66295-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66295-1-Ig (P-cadherin antibody) at dilution of 1:250 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66295-1-PBS in a different storage buffer formulation.

  • FC experiment of A431 using 66295-1-Ig (same clone as 66295-1-PBS)

    FC experiment of A431 using 66295-1-Ig (same clone as 66295-1-PBS)

    1X10^6 A431 cells were stained with 0.20ug P-cadherin antibody (66295-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH. This data was developed using the same antibody clone with 66295-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
Sample-dependent, check data in validation data gallery

Product Information

66295-1-PBS targets P-cadherin in WB, FC, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen P-cadherin fusion protein Ag4758 相同性解析による交差性が予測される生物種
Full Name cadherin 3, type 1, P-cadherin (placental)
Calculated molecular weight 829 aa, 91 kDa
Observed molecular weight 120 kDa
GenBank accession numberBC041846
Gene symbol CDH3
Gene ID (NCBI) 1001
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. P-cadherin (placental cadherin), also known as CDH3 (cadherin 3), is a classical member of the cadherin superfamily which also include E-, N-, R-, and B-cadherins. Aberrant expression of P-cadherin has been reported in many cancers, including breast, gastric, endometrial, ovarian, and prostate cancer.