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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using P-selectin / CD62P Monoclonal antibody (60322-1-Ig)

    WB analysis using 60322-1-Ig (same clone as 60322-1-PBS)

    Untreated and PNGase F-treated lysates of human platelets were subjected to SDS PAGE followed by western blot with 60322-1-Ig (P-selectin / CD62P antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808). This data was developed using the same antibody clone with 60322-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human peripheral blood platelets using P-selectin / CD62P Monoclonal antibody (60322-1-Ig)

    WB analysis of human peripheral blood platelets using 60322-1-Ig (same clone as 60322-1-PBS)

    human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 60322-1-Ig (P-selectin / CD62P antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60322-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using P-selectin / CD62P Monoclonal antibody (60322-1-Ig)

    WB analysis of Jurkat using 60322-1-Ig (same clone as 60322-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 60322-1-Ig (P-selectin / CD62P antibody at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60322-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of K-562 cells using P-selectin / CD62P Monoclonal antibody (60322-1-Ig)

    IP experiment of K-562 using 60322-1-Ig (same clone as 60322-1-PBS)

    IP result of anti-P-selectin / CD62P (IP:60322-1-Ig, 5ug; Detection:60322-1-Ig 1:600) with K-562 cells lysate 1760ug. This data was developed using the same antibody clone with 60322-1-PBS in a different storage buffer formulation.

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P-selectin / CD62P Monoclonal antibody, PBS Only

P-selectin / CD62P Monoclonal Antibody for WB, IP, Indirect ELISA
Cat No. 60322-1-PBS
Clone No.3G2C7

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IP, Indirect ELISA

Synonyms

CD62P, P-selectin, GMP140, GMP 140, CD62 antigen-like family member P

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60322-1-PBS targets P-selectin / CD62P in WB, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen P-selectin / CD62P fusion protein Ag4124 相同性解析による交差性が予測される生物種
Full Name selectin P (granule membrane protein 140kDa, antigen CD62)
Calculated molecular weight 830 aa, 91 kDa
Observed molecular weight 140 kDa, 105-110 kDa
GenBank accession numberBC028067
Gene Symbol CD62P
Gene ID (NCBI) 6403
RRIDAB_2881433
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP16109
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

P-selectin, also known as GMP140 or CD62P, is a transmembrane glycoprotein that mediates the interaction of activated endothelial cells or platelets with leukocytes. It is an adhesion molecule involved in the pathogenesis of inflammation, thrombosis, and oncogenesis. P-selectin is stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. Upon cell activation by agonists, P-selectin is transported rapidly to the cell surface.