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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PP11 Polyclonal antibody

PP11 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 18002-1-AP

Synonyms

26 serine protease, ENDOU, P11, Placental protein 11, PP11, Protein endoU, PRSS26

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse lung tissue using PP11 Polyclonal antibody (18002-1-AP)

    WB analysis of mouse lung using 18002-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 18002-1-AP (PP11 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using PP11 Polyclonal antibody (18002-1-AP)

    WB analysis of HeLa using 18002-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 18002-1-AP (PP11 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PP11 Polyclonal antibody (18002-1-AP)

    IHC staining of human breast cancer using 18002-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18002-1-AP (PP11 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PP11 Polyclonal antibody (18002-1-AP)

    IHC staining of human breast cancer using 18002-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18002-1-AP (PP11 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PP11 Polyclonal antibody (18002-1-AP)

    IF Staining of HeLa using 18002-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 18002-1-AP (PP11 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inmouse lung tissue, HeLa cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 2 publications below

Product Information

18002-1-AP targets PP11 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PP11 fusion protein Ag12494 相同性解析による交差性が予測される生物種
Full Name 26 serine protease
Calculated molecular weight 369 aa, 42 kDa
Observed molecular weight 35-40 kDa
GenBank accession numberBC074763
Gene symbol PP11
Gene ID (NCBI) 8909
RRIDAB_10598470
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PP11 antibody 18002-1-APDownload protocol
IHC protocol for PP11 antibody 18002-1-APDownload protocol
IF protocol for PP11 antibody 18002-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Am J Obstet Gynecol

Trophoblast apoptosis from pregnancies complicated by fetal growth restriction is associated with enhanced p53 expression.

Authors - Roni Levy
View Article
humanIHC

J Clin Biochem Nutr

Upregulation of ENDOU in cytotrophoblasts from placenta complicated with preeclampsia and fetal growth restriction.

Authors - Masataka Nomoto
View Article