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Species Reactivity
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

P16-INK4A Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: human

Detection: human

Applications

Sandwich ELISA

Range

Sandwich ELISA: 0.195-12.5 ng/mL

Cat No : MP01135-4

Print datasheet

Synonyms

Validation Data Gallery

  • Sandwich ELISA standard curve of MP01135-4, Human P16-INK4A Recombinant Matched Antibody Pair - PBS only. 81373-6-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag1328. 81373-4-PBS was HRP conjugated as the detection antibody. Range: 0.195-12.5 ng/mL.

    Sandwich ELISA standard curve of MP01135-4

    Sandwich ELISA standard curve of MP01135-4, Human P16-INK4A Recombinant Matched Antibody Pair - PBS only. 81373-6-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag1328. 81373-4-PBS was HRP conjugated as the detection antibody. Range: 0.195-12.5 ng/mL.

  • The mean P16-INK4A concentration was determined to be 39.21 ng/mL in HeLa cell extract based on a 2.80 mg/mL extract load.

    Sample test of MP01135-4

    The mean P16-INK4A concentration was determined to be 39.21 ng/mL in HeLa cell extract based on a 2.80 mg/mL extract load.

Tested Applications

Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

MP01135-4 targets P16-INK4A in immunoassays as a matched antibody pair. Validated in Sandwich ELISA.

Range: 0.195-12.5 ng/mL (Sandwich ELISA);

Capture antibody: P16-INK4A Recombinant antibody, PBS Only (Capture) 81373-6-PBS (241438G3). 100 μg. Concentration 1 mgl/ml.

Detection antibody: P16-INK4A Recombinant antibody, PBS Only (Detector) 81373-4-PBS (241438D6). 100 μg. Concentration 1 mgl/ml.

Alternative P16-INK4A matched antibody pairs: MP01135-1, MP01135-2, MP01135-3

Matched pair protein standard: Ag1328

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 81373-6-PBS
Tested Reactivity human
Immunogen P16-INK4A fusion protein Ag1328
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4)
Synonyms CDKN2A, MTS1, CDK4I, CDK4 inhibitor p16INK4, CDK4 inhibitor p16 INK4
GenBank accession numberBC021998
Gene symbol P16-INK4A
UniprotQ8N726
Gene ID (NCBI) 1029
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
Catalog Number 81373-4-PBS
Tested Reactivity human
Immunogen P16-INK4A fusion protein Ag1328
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4)
Synonyms CDKN2A, MTS1, CDK4I, CDK4 inhibitor p16INK4, CDK4 inhibitor p16 INK4
GenBank accession numberBC021998
Gene symbol P16-INK4A
UniprotQ8N726
Gene ID (NCBI) 1029
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.