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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

P21 Recombinant antibody, PBS Only

P21 Recombinant Antibody for WB, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3N14

Cat no : 82669-2-PBS

Synonyms

CDKN1A, P21;CDKN1A, Cyclin-dependent kinase inhibitor 1, Cyclin dependent kinase inhibitor 1, CIP1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using P21 Recombinant antibody (82669-2-RR)

    WB analysis using 82669-2-RR (same clone as 82669-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82669-2-RR (P21 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82669-2-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of MCF-7 cells using P21 Recombinant antibody (82669-2-RR)

    IP experiment of MCF-7 using 82669-2-RR (same clone as 82669-2-PBS)

    IP result of anti-P21 (IP:82669-2-RR, 4ug; Detection:82669-2-RR 1:800) with MCF-7 cells lysate 760 ug. This data was developed using the same antibody clone with 82669-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using P21 Recombinant antibody (82669-2-RR)

    IF Staining of MCF-7 using 82669-2-RR (same clone as 82669-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using P21 antibody (82669-2-RR, Clone: 3N14 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82669-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82669-2-RR

    Affinity and Kinetic Characterization of 82669-2-RR

    Biolayer interferometry (BLl) kinetic assays of 82669-2-RR against Human P21 were performed. The affinity constant is 112 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82669-2-PBS targets P21 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen P21 fusion protein Ag0368 相同性解析による交差性が予測される生物種
Full Name cyclin-dependent kinase inhibitor 1A (p21, Cip1)
Calculated molecular weight18 kDa
Observed molecular weight21 kDa
GenBank accession numberBC000275
Gene symbol p21
Gene ID (NCBI) 1026
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CDKN1A (p21, CIP1, WAF1) is a cyclin-dependent kinase inhibitor. CDKN1A binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at the G1 phase. The expression of CDKN1A is induced by wild-type but not mutant p53 protein, through which CDKN1A mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. CDKN1A can interact with proliferating cell nuclear antigen (PCNA), and plays a regulatory role in S phase DNA replication and DNA damage repair. CDKN1A was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Two alternatively spliced variants, which encode an identical protein, have been reported.