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  • Featured Product
  • KD/KO Validated

P27; KIP1 Polyclonal antibody

P27; KIP1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

Cat no : 26714-1-AP

Synonyms

CDKN1B, P27, MEN1B, KIP1, Cyclin-dependent kinase inhibitor p27

Validation Data Gallery

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  • Western Blot (WB) analysis of NIH/3T3 cells using P27; KIP1 Polyclonal antibody (26714-1-AP)

    WB analysis of NIH/3T3 using 26714-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 26714-1-AP (P27; KIP1 Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using P27; KIP1 Polyclonal antibody (26714-1-AP)

    WB analysis of HeLa using 26714-1-AP

    WB result of P27; KIP1 antibody (26714-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-P27; KIP1 transfected HeLa cells.

  • Western Blot (WB) analysis of MCF-7 cells using P27; KIP1 Polyclonal antibody (26714-1-AP)

    WB analysis of MCF-7 using 26714-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 26714-1-AP (P27; KIP1 Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using P27; KIP1 Polyclonal antibody (26714-1-AP)

    IHC staining of human gliomas using 26714-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 26714-1-AP (P27; KIP1 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human gliomas tissue using P27; KIP1 Polyclonal antibody (26714-1-AP)

    IHC staining of human gliomas using 26714-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 26714-1-AP (P27; KIP1 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using P27; KIP1 Polyclonal antibody (26714-1-AP)

    IHC staining of human ovary tumor using 26714-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 26714-1-AP (P27; KIP1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using P27; KIP1 Polyclonal antibody (26714-1-AP)

    IHC staining of human tonsillitis using 26714-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 26714-1-AP (P27; KIP1 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using P27; KIP1 Polyclonal antibody (26714-1-AP)

    IHC staining of human tonsillitis using 26714-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 26714-1-AP (P27; KIP1 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using P27; KIP1 Polyclonal antibody (26714-1-AP)

    IHC staining of human lung cancer using 26714-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26714-1-AP (P27; KIP1 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using P27; KIP1 Polyclonal antibody (26714-1-AP)

    IHC staining of human lung cancer using 26714-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26714-1-AP (P27; KIP1 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using P27; KIP1 Polyclonal antibody (26714-1-AP)

    IHC staining of human breast cancer using 26714-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26714-1-AP (P27; KIP1 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using P27; KIP1 Polyclonal antibody (26714-1-AP)

    IHC staining of human breast cancer using 26714-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26714-1-AP (P27; KIP1 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using P27; KIP1 Polyclonal antibody (26714-1-AP)

    IHC staining of human colon cancer using 26714-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26714-1-AP (P27; KIP1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using P27; KIP1 Polyclonal antibody (26714-1-AP)

    IHC staining of human colon cancer using 26714-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26714-1-AP (P27; KIP1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using P27; KIP1 Polyclonal antibody (26714-1-AP)

    IHC staining of human ovary tumor using 26714-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 26714-1-AP (P27; KIP1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using P27; KIP1 Polyclonal antibody (26714-1-AP)

    IF Staining of MCF-7 using 26714-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 26714-1-AP (P27; KIP1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using P27; KIP1 Polyclonal antibody (26714-1-AP)

    IF Staining of HepG2 using 26714-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using P27; KIP1 antibody (26714-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

  • Flow cytometry (FC) experiment of MCF-7 cells using P27; KIP1 Polyclonal antibody (26714-1-AP)

    FC experiment of MCF-7 using 26714-1-AP

    1X10^6 MCF-7 cells were intracellularly stained with 0.2 ug Anti-Human P27; KIP1 (26714-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

Positive WB detected inNIH/3T3 cells, MCF-7 cells, HeLa cells
Positive IHC detected inhuman gliomas tissue, human tonsillitis tissue, human lung cancer tissue, human breast cancer tissue, human colon cancer tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, MCF-7 cells
Positive FC (Intra) detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

26714-1-AP targets P27; KIP1 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen P27; KIP1 fusion protein Ag25083 相同性解析による交差性が予測される生物種
Full Name cyclin-dependent kinase inhibitor 1B (p27, Kip1)
Calculated molecular weight 198 aa, 22 kDa
Observed molecular weight 27 kDa
GenBank accession numberBC001971
Gene symbol p27 Kip1/CDKN1B
Gene ID (NCBI) 1027
RRIDAB_2880611
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CDKN1B, also named as P27 or KIP1, is a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. P27 binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controlling cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Downregulation of P27 has been implicated in the progression of several malignancies, including lung cancer, hepatocellular carcinoma, salivary cancer, oral squamous cell carcinomas, and gastric cancer.

Protocols

Product Specific Protocols
WB protocol for P27; KIP1 antibody 26714-1-APDownload protocol
IHC protocol for P27; KIP1 antibody 26714-1-APDownload protocol
IF protocol for P27; KIP1 antibody 26714-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Int J Oncol

Effects of three-dimensional collagen scaffolds on the expression profiles and biological functions of glioma cells.

Authors - Wei Jia
View Article
humanWB

Biosci Rep

C1QTNF6 regulates cell proliferation and apoptosis of NSCLC in vitro and in vivo.

Authors - Wei Zhang
View Article